RGD Reference Report - Antinociceptive potentiation and attenuation of tolerance by intrathecal {beta}-arrestin 2 small interfering RNA in rats. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Antinociceptive potentiation and attenuation of tolerance by intrathecal {beta}-arrestin 2 small interfering RNA in rats.

Authors: Yang, CH  Huang, HW  Chen, KH  Chen, YS  Sheen-Chen, SM  Lin, CR 
Citation: Yang CH, etal., Br J Anaesth. 2011 Nov;107(5):774-81. Epub 2011 Sep 17.
RGD ID: 5509871
Pubmed: PMID:21926413   (View Abstract at PubMed)
DOI: DOI:10.1093/bja/aer291   (Journal Full-text)

BACKGROUND: /st> Tolerance to the analgesic effect of opioids complicates the management of persistent pain states. We tested whether the intrathecal infusion of small interfering RNA (siRNA) against beta-arrestin 2 would reduce tolerance to chronic morphine use and the severity of precipitated morphine withdrawal. METHODS: /st> Intrathecal beta-arrestin 2 (2 mug siRNA per 10 mul per rat) was injected once daily for 3 days. Rats then received a continuous intrathecal infusion of morphine (2 nmol h(-1)) or saline for 7 days. Daily tail-flick (TF) and intrathecal morphine challenge tests were performed to assess the effect of intrathecal beta-arrestin 2 siRNA on antinociception and tolerance to morphine. Naloxone withdrawal (2 mg kg(-1)) was performed to assess morphine dependence. RESULTS: /st> In the daily TF test, the antinociception of intrathecal morphine was increased and maintained in rats receiving beta-arrestin 2 siRNA compared with the control group (morphine alone). In the probe response test, rats receiving morphine infusion with beta-arrestin 2 siRNA treatment showed a significant left shift in their dose-response curve, as measured by per cent maximal possible effect (MPE), such that the AD(50) was significantly decreased by a factor of 5.6 when compared with that of morphine-infused rats. In the naloxone-induced withdrawal tests, rats receiving beta-arrestin 2 siRNA injection with morphine infusion showed a significant reduction in four of the six signs of withdrawal. CONCLUSIONS: /st> We show here that intrathecal beta-arrestin 2 siRNA in rats enhances analgesia and attenuates naloxone-induced withdrawal symptoms. This may warrant further investigation in the context of long-term use of intrathecal opioids for controlling chronic pain.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
detection of temperature stimulus involved in sensory perception of pain  IMP 5509871 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Arrb2  (arrestin, beta 2)


Additional Information