RGD Reference Report - Two-dimensional difference gel electrophoresis (DiGE) analysis of plasmas from dengue fever patients. - Rat Genome Database

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Two-dimensional difference gel electrophoresis (DiGE) analysis of plasmas from dengue fever patients.

Authors: Albuquerque, LM  Trugilho, MR  Chapeaurouge, A  Jurgilas, PB  Bozza, PT  Bozza, FA  Perales, J  Neves-Ferreira, AG 
Citation: Albuquerque LM, etal., J Proteome Res. 2009 Dec;8(12):5431-41.
RGD ID: 5509870
Pubmed: (View Article at PubMed) PMID:19845402
DOI: Full-text: DOI:10.1021/pr900236f

Dengue fever is the world's most important arthropod-born viral disease affecting humans. To contribute to a better understanding of its pathogenesis, this study aims to identify proteins differentially expressed in plasmas from severe dengue fever patients relative to healthy donors. The use of 2-D Fluorescence Difference Gel Electrophoresis to analyze plasmas depleted of six high-abundance proteins (albumin, IgG, antitrypsin, IgA, transferrin and haptoglobin) allowed for the detection of 73 differentially expressed protein spots (n = 13, p < 0.01), of which 37 could be identified by mass spectrometry. These 37 spots comprised a total of 14 proteins, as follows: 7 had increased expression in plasmas from dengue fever patients (C1 inhibitor, alpha1-antichymotrypsin, vitamin D-binding protein, fibrinogen gamma-chain, alpha1-acid glycoprotein, apolipoprotein J and complement component C3c), while 7 others had decreased expression in the same samples (alpha-2 macroglobulin, prothrombin, histidine-rich glycoprotein, apolipoproteins A-IV and A-I, transthyretin and complement component C3b). The possible involvement of these proteins in the inflammatory process triggered by dengue virus infection and in the repair mechanisms of vascular damage occurring in this pathology is discussed in this study.

Annotation

Disease Annotations    
dengue disease  (IEP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Gc  (GC, vitamin D binding protein)

Genes (Mus musculus)
Gc  (vitamin D binding protein)

Genes (Homo sapiens)
GC  (GC vitamin D binding protein)


Additional Information