RGD Reference Report - Proteomic identification of biomarkers of vascular injury. - Rat Genome Database

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Proteomic identification of biomarkers of vascular injury.

Authors: Huang, NF  Kurpinski, K  Fang, Q  Lee, RJ  Li, S 
Citation: Huang NF, etal., Am J Transl Res. 2011 Feb;3(2):139-48. Epub 2010 Nov 21.
RGD ID: 5509868
Pubmed: PMID:21416056   (View Abstract at PubMed)
PMCID: PMC3056560   (View Article at PubMed Central)

Predictive biomarkers may be beneficial for detecting, diagnosing, and assessing the risk of restenosis and vascular injury. We utilized proteomic profiling to identify protein markers in the blood following vascular injury, and corroborated the differential protein expression with immunological approaches. Rats underwent carotid artery injury, and plasma was collected after 2 or 5 weeks. Proteomic profiling was carried out by two-dimensional differential in-gel electrophoresis. The differentially expressed plasma proteins were identified by mass spectroscopy and confirmed by immunoblotting. Proteomic profiling by two-dimensional differential in-gel electrophoresis and mass spectroscopy revealed plasma proteins that were differentially expressed at 2 weeks after injury. Among the proteins identified included vitamin D binding protein (VDBP), aldolase A (aldo A), and apolipoproteinE (apoE). Immunoblotting results validated a significant reduction in these proteins in the plasma at 2 or 5 weeks after vascular injury, in comparison to control animals without vascular injury. These findings suggest that VDBP, aldo A, and apoE may be biomarkers for vascular injury, which will have important prognostic and diagnostic implications.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GCHumanCarotid Artery Injuries  ISOGc (Rattus norvegicus)protein:decreased expression:plasmaRGD 
GcRatCarotid Artery Injuries  IEP protein:decreased expression:plasmaRGD 
GcMouseCarotid Artery Injuries  ISOGc (Rattus norvegicus)protein:decreased expression:plasmaRGD 
APOEHumanVascular System Injuries  ISOApoe (Rattus norvegicus)protein:decreased expression:plasma (rat)RGD 
ApoeRatVascular System Injuries  IEP protein:decreased expression:plasma (rat)RGD 
ApoeMouseVascular System Injuries  ISOApoe (Rattus norvegicus)protein:decreased expression:plasma (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Apoe  (apolipoprotein E)
Gc  (GC, vitamin D binding protein)

Genes (Mus musculus)
Apoe  (apolipoprotein E)
Gc  (vitamin D binding protein)

Genes (Homo sapiens)
APOE  (apolipoprotein E)
GC  (GC vitamin D binding protein)


Additional Information