RGD Reference Report - [Comparative proteomic analysis of cerebral cortex between middle cerebral artery occlusion rats and normal controls]. - Rat Genome Database
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[Comparative proteomic analysis of cerebral cortex between middle cerebral artery occlusion rats and normal controls].

Authors: Zhao, Xiao-Feng  Wen, Jing-Rong  Wang, Shu  Shi, Xue-Min 
Citation: Zhao XF, etal., Sheng Wu Gong Cheng Xue Bao. 2005 Nov;21(6):934-41.
RGD ID: 5508760
Pubmed: (View Article at PubMed) PMID:16468349

In order to provide a complete picture of pathogenesis in cerebral ischemia, cerebral cortex in MCAO rats were analysed for alteration in their proteomes. Comparative proteome analysis was used to compare signal corresponding to individual cerebral cortex proteins on a two-dimensional gel between MCAO rats and the normal control (NC) group. After sample preparation, two-dimensional electroghoresis separated proteins were stained with Commassie Brilliant Blue. The image data were analyzed on a Dell computer using Image Master v 3.01 software. In cerebral cortex, 30 proteins were differentially expressed in MCAO rats compared with NC. There were 11 spots significantly increased, 15 spots significantly decreased and Adenylate kinase isoenzyme 1 was detected only in NC group, biliverdin reductase B, small inducible cytokine A4 [Precursor] only in MCAO group. Peroxiredoxin 2 divided into two points in MCAO6h group. In the end, this approach may lay a foundation for the further investigation of pathogenic mechanisms in cerebral ischmic injury.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Ak1  (adenylate kinase 1)

Genes (Mus musculus)
Ak1  (adenylate kinase 1)

Genes (Homo sapiens)
AK1  (adenylate kinase 1)


Additional Information