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A NOD2 gene polymorphism is associated with the prevalence and severity of chronic obstructive pulmonary disease in a Japanese population.

Authors: Kinose, D  Ogawa, E  Hirota, T  Ito, I  Kudo, M  Haruna, A  Marumo, S  Hoshino, Y  Muro, S  Hirai, T  Sakai, H  Date, H  Tamari, M  Mishima, M 
Citation: Kinose D, etal., Respirology. 2011 Sep 22. doi: 10.1111/j.1440-1843.2011.02069.x.
Pubmed: (View Article at PubMed) PMID:21943069
DOI: Full-text: DOI:10.1111/j.1440-1843.2011.02069.x

SUMMARY AT A GLANCE: This study demonstrated that variation in the NOD2 gene was associated with COPD and also with the severity of COPD. Dysfunction of NOD2 due to this variation may be another important factor in the host-pathogen relationship and clinical course of COPD. ABSTRACT: Background and objective: Genetic background is thought to be one of the risk factors for development of COPD. Recently, it has been proposed that the innate immune system is involved in the pathophysiology of COPD. We hypothesized that polymorphisms in the nucleotide-binding and oligomerization domain (NOD)1 and NOD2 genes would be associated with the pathogenesis of COPD. In addition, the associations between these single nucleotide polymorphisms (SNPs) and phenotypes of COPD were analyzed. Methods: Japanese COPD patients (n= 228) and non-COPD smokers (n= 101) were recruited from the outpatient clinic at Kyoto University Hospital, Kyoto, Japan. At entry into the study, a blood sample was taken and a pulmonary function test was performed. Genotyping was performed for six selected tag SNPs of NOD1 and five tag SNPs of NOD2. Further investigations were performed for SNPs that were associated with COPD, including baseline gene expression, the relative proportions of splicing variants in whole blood, responses to ligand and enhancement of gene expression in peripheral blood neutrophils stimulated with pro-inflammatory cytokines. Results: The distribution of NOD2 rs1077861 genotypes differed between Japanese COPD patients and non-COPD smokers (P= 0.036). This SNP was also associated with a lower FEV(1) % predicted (57.2 +/- 1.8 for TT vs. 50.8 +/- 2.3 for TA/AA, P= 0.03) and DL(CO) /V(A) (2.89 +/- 0.1 in TT vs. 2.53 +/- 0.14 in TA/AA, P= 0.036) in COPD patients. NOD2 gene expression after stimulation with 10 ng/mL of TNF-alpha for 4 h, was increased to a greater extent in TA/AA genotype than in TT genotype peripheral blood neutrophils (P= 0.015). Conclusions: The NOD2 rs1077861 SNP may influence the development and progression of COPD in Japanese subjects.

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RGD Object Information
RGD ID: 5508720
Created: 2011-10-20
Species: All species
Last Modified: 2011-10-20
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.