RGD Reference Report - Novel mutations of mitochondrial complex I in pathologically proven Parkinson disease. - Rat Genome Database
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Novel mutations of mitochondrial complex I in pathologically proven Parkinson disease.

Authors: Kosel, S  Grasbon-Frodl, EM  Mautsch, U  Egensperger, R  Von Eitzen, U  Frishman, D  Hofmann, S  Gerbitz, KD  Mehraein, P  Graeber, MB 
Citation: Kosel S, etal., Neurogenetics. 1998 Mar;1(3):197-204.
RGD ID: 5507832
Pubmed: (View Article at PubMed) PMID:10737123

Complete sequence analysis of all mitochondrial complex I genes was performed in 22 cases of neuropathologically confirmed idiopathic Parkinson disease (PD). DNA from the substantia nigra was used as a template for polymerase chain reaction-based genomic sequencing. Seven novel mutations causing the exchange of amino acids were detected in subunit genes ND1 (3992 C/ T, 4024 A/G), ND4 (11253 T/C, 12084 C/T), ND5 (13711 G/A, 13768 T/C), and ND6 (14582 T/C). In addition, five known missense mutations affecting the ND1 (3335 T/C, 3338 T/C), ND2 (5460 G/A), ND3 (10398 A/G), and ND5 (13966 A/G) genes as well as three secondary LHON mutations (4216 T/C, 4917 A/ G, 13708 G/A) were found in the PD group. Among the novel mutations, the 11253 T/C transition which changes a conserved isoleucine residue into threonine is most likely to be of functional relevance. Furthermore, 43 synonymous polymorphisms were detected in PD brains, including 20 novel sequence variants. Haplogroup analysis revealed that most unique missense mutations were found in PD cases belonging to the D(c) haplogroup. Our data are in line with the view that PD is not a single disease entity but comprises a genetically heterogeneous group of disorders. The results of our study further suggest that 90% or more of all idiopathic PD cases are not due to sequence variation of mitochondrial complex I, but that mitochondrial mutations may play a pathogenic role in a subset of PD patients.

Annotation

Disease Annotations    
Parkinson's disease  (IAGP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Mt-nd2  (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 2)
Mt-nd4  (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 4)

Genes (Mus musculus)
mt-Nd2  (NADH dehydrogenase 2, mitochondrial)
mt-Nd4  (NADH dehydrogenase 4, mitochondrial)

Genes (Homo sapiens)
MT-ND2  (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 2)
MT-ND4  (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 4)


Additional Information