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Differential gene expression pattern of diabetic rat retinas after intravitreal injection of erythropoietin.

Authors: Chu, Q  Zhang, J  Wu, Y  Zhang, Y  Xu, G  Li, W  Xu, GT 
Citation: Chu Q, etal., Clin Experiment Ophthalmol. 2011 Mar;39(2):142-51. doi: 10.1111/j.1442-9071.2010.02437.x.
Pubmed: (View Article at PubMed) PMID:20973890
DOI: Full-text: DOI:10.1111/j.1442-9071.2010.02437.x

BACKGROUND: To profile the pattern of gene expression in diabetic rat retinas with or without intravitreal injection of erythropoietin. DESIGN: By using streptozotocin-induced diabetic rats, after intravitreal injection of erythropoietin, neurosensory retinas were collected to determine the effect of erythropoietin on gene expression. PARTICIPANTS: Three groups of Sprague-Dawley rats were studied: normal control (15), diabetic rats with saline injection (15) and diabetic rats with intravitreal erythropoietin treatment (15). METHODS: Diabetes was induced by intra-peritoneal injection of streptozotocin. Intravitreal injection of erythropoietin was performed at the following time points: 0, 30 and 120 days after diabetes onset. Four days after each injection at above-mentioned time points, the retinas were harvested for microarray assay. The real-time PCR was used to evaluate the microarray data. RESULTS: Genes encoding inflammatory factors, such as interleukin-2 and interleukin-11, which were upregulated in the diabetic retinas, were restored after erythropoietin treatment. Genes encoding pro-apoptotic effectors, like Tnfrsf5, Bid3 and Bcl2l1, were also upregulated in diabetic rats and attenuated in erythropoietin-treated group. In addition, real-time PCR were employed to confirm the changes of the genes Trex2, G1P2, DHX58, RGD1311906 and LOC689064, which have not been reported in diabetic retinopathy. CONCLUSIONS: Intravitreal erythropoietin treatment is able to normalize the gene expression responsible for pro-apoptotic and inflammatory responses noted in diabetic retinas.

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RGD ID: 5490977
Created: 2011-09-21
Species: All species
Last Modified: 2011-09-21
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.