RGD Reference Report - Elucidating the molecular physiopathology of acute respiratory distress syndrome in severe acute respiratory syndrome patients. - Rat Genome Database

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Elucidating the molecular physiopathology of acute respiratory distress syndrome in severe acute respiratory syndrome patients.

Authors: Kong, SL  Chui, P  Lim, B  Salto-Tellez, M 
Citation: Kong SL, etal., Virus Res. 2009 Nov;145(2):260-9. Epub 2009 Jul 25.
RGD ID: 5490168
Pubmed: PMID:19635508   (View Abstract at PubMed)
PMCID: PMC7114434   (View Article at PubMed Central)
DOI: DOI:10.1016/j.virusres.2009.07.014   (Journal Full-text)

Acute respiratory distress syndrome (ARDS) is a severe form of acute lung injury. It is a response to various diseases of variable etiology, including SARS-CoV infection. To date, a comprehensive study of the genomic physiopathology of ARDS (and SARS) is lacking, primarily due to the difficulty of finding suitable materials to study the disease process at a tissue level (instead of blood, sputa or swaps). Hereby we attempt to provide such study by analyzing autopsy lung samples from patient who died of SARS and showed different degrees of severity of the pulmonary involvement. We performed real-time quantitative PCR analysis of 107 genes with functional roles in inflammation, coagulation, fibrosis and apoptosis; some key genes were confirmed at a protein expression level by immunohistochemistry and correlated to the degree of morphological severity present in the individual samples analyzed. Significant expression levels were identified for ANPEP (a receptor for CoV), as well as inhibition of the STAT1 pathway, IFNs production and CXCL10 (a T-cell recruiter). Other genes unassociated to date with ARDS/SARS include C1Qb, C5R1, CASP3, CASP9, CD14, CD68, FGF7, HLA-DRA, IGF1, IRF3, MALAT-1, MSR1, NFIL3, SLPI, USP33, CLC, GBP1 and TAC1. As a result, we proposed to therapeutically target some of these genes with compounds such as ANPEP inhibitors, SLPI and dexamethasone. Ultimately, this study may serve as a model for future, tissue-based analyses of fibroinflammatory conditions affecting the lung.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
severe acute respiratory syndrome disease_progressionIEP 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOANPEP (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOBPI (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOC5AR1 (Homo sapiens)5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionIEP 5490168; 5490168; 5490168mRNA and protein:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionIEP 5490168; 5490168; 5490168mRNA:decreased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOCASP3 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOCASP9 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOCD14 (Homo sapiens)5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOCD4 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOCD68 (Homo sapiens)5490168; 5490168mRNA and protein:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOCD9 (Homo sapiens)5490168; 5490168mRNA and protein:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOCLCF1 (Homo sapiens)5490168; 5490168mRNA:decreased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOCXCL10 (Homo sapiens)5490168; 5490168mRNA:decreased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOFGF7 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOHLA-DRA (Homo sapiens)5490168; 5490168mRNA:increased expression:lungRGD 
severe acute respiratory syndrome disease_progressionIEP 5490168mRNA:increased expression:lungRGD 
severe acute respiratory syndrome disease_progressionISOHP (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOIGF1 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOIL1R1 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOIRF3 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOITGA2B (Homo sapiens)5490168; 5490168mRNA:decreased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOMSR1 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISONFIL3 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOPECAM1 (Homo sapiens)5490168; 5490168mRNA and protein:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISORAB31 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOS100A8 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOSLPI (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 
severe acute respiratory syndrome disease_progressionISOTLR2 (Homo sapiens)5490168; 5490168mRNA:increased expression:lung (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Anpep  (alanyl aminopeptidase, membrane)
Bpi  (bactericidal/permeability-increasing protein)
Casp3  (caspase 3)
Casp9  (caspase 9)
Cd4  (Cd4 molecule)
Cd68  (Cd68 molecule)
Cd9  (CD9 molecule)
Clcf1  (cardiotrophin-like cytokine factor 1)
Cxcl10  (C-X-C motif chemokine ligand 10)
Fgf7  (fibroblast growth factor 7)
Hp  (haptoglobin)
Igf1  (insulin-like growth factor 1)
Il1r1  (interleukin 1 receptor type 1)
Irf3  (interferon regulatory factor 3)
Itga2b  (integrin subunit alpha 2b)
Msr1  (macrophage scavenger receptor 1)
Nfil3  (nuclear factor, interleukin 3 regulated)
Pecam1  (platelet and endothelial cell adhesion molecule 1)
Rab31  (RAB31, member RAS oncogene family)
RT1-Da  (RT1 class II, locus Da)
S100a8  (S100 calcium binding protein A8)
Slpi  (secretory leukocyte peptidase inhibitor)
Tlr2  (toll-like receptor 2)

Genes (Mus musculus)
Anpep  (alanyl aminopeptidase, membrane)
Bpi  (bactericidal permeablility increasing protein)
C5ar1  (complement component 5a receptor 1)
Casp3  (caspase 3)
Casp9  (caspase 9)
Cd14  (CD14 antigen)
Cd4  (CD4 antigen)
Cd68  (CD68 antigen)
Cd9  (CD9 antigen)
Clcf1  (cardiotrophin-like cytokine factor 1)
Cxcl10  (C-X-C motif chemokine ligand 10)
Fgf7  (fibroblast growth factor 7)
H2-Ea  (histocompatibility 2, class II antigen E alpha)
Hp  (haptoglobin)
Igf1  (insulin-like growth factor 1)
Il1r1  (interleukin 1 receptor, type I)
Irf3  (interferon regulatory factor 3)
Itga2b  (integrin alpha 2b)
Msr1  (macrophage scavenger receptor 1)
Nfil3  (nuclear factor, interleukin 3, regulated)
Pecam1  (platelet/endothelial cell adhesion molecule 1)
Rab31  (RAB31, member RAS oncogene family)
S100a8  (S100 calcium binding protein A8 (calgranulin A))
Slpi  (secretory leukocyte peptidase inhibitor)
Tlr2  (toll-like receptor 2)

Genes (Homo sapiens)
ANPEP  (alanyl aminopeptidase, membrane)
BPI  (bactericidal permeability increasing protein)
C1QB  (complement C1q B chain)
C5AR1  (complement C5a receptor 1)
CASP3  (caspase 3)
CASP9  (caspase 9)
CD14  (CD14 molecule)
CD4  (CD4 molecule)
CD68  (CD68 molecule)
CD9  (CD9 molecule)
CEACAM8  (CEA cell adhesion molecule 8)
CLCF1  (cardiotrophin like cytokine factor 1)
CXCL10  (C-X-C motif chemokine ligand 10)
CXCL8  (C-X-C motif chemokine ligand 8)
FGF7  (fibroblast growth factor 7)
HLA-DRA  (major histocompatibility complex, class II, DR alpha)
HP  (haptoglobin)
IGF1  (insulin like growth factor 1)
IL1R1  (interleukin 1 receptor type 1)
IRF3  (interferon regulatory factor 3)
ITGA2B  (integrin subunit alpha 2b)
MSR1  (macrophage scavenger receptor 1)
NFIL3  (nuclear factor, interleukin 3 regulated)
PECAM1  (platelet and endothelial cell adhesion molecule 1)
RAB31  (RAB31, member RAS oncogene family)
S100A8  (S100 calcium binding protein A8)
SLPI  (secretory leukocyte peptidase inhibitor)
TLR2  (toll like receptor 2)


Additional Information