RGD Reference Report - Effects of intravitreal triamcinolone acetonide on retinal gene expression in a rat model of central retinal vein occlusion. - Rat Genome Database

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Effects of intravitreal triamcinolone acetonide on retinal gene expression in a rat model of central retinal vein occlusion.

Authors: Rehak, M  Drechsler, F  Koferl, P  Hollborn, M  Wiedemann, P  Bringmann, A  Kohen, L 
Citation: Rehak M, etal., Graefes Arch Clin Exp Ophthalmol. 2011 Aug;249(8):1175-83. Epub 2011 Apr 13.
RGD ID: 5490120
Pubmed: (View Article at PubMed) PMID:21487926
DOI: Full-text: DOI:10.1007/s00417-011-1683-z

PURPOSE: The aim of this study was to investigate the effects of intravitreal triamcinolone acetonide on the alterations in retinal gene expression in a rat model of central retinal vein occlusion (CRVO). METHODS: In one eye of adult Brown Norway rats (n = 77) CRVO was induced with laser photocoagulation of all retinal veins near to the optic disk after intraperitoneal injection of 0.2 ml of 10% sodium fluorescein. The gene expression was investigated using RT-PCR separately in the neural retina and retinal pigment epithelium (RPE) 1, 3, 7, and 14 days after CRVO induction. We analyzed the expression of factors that influence the development of vascular edema (VEGF-A, VEGF-B, PEDF), of channels implicated in retinal osmohomeostasis (Kir4.1, AQP4, AQP1), and of the pro-inflammatory factors IL-1ss and IL-6. RESULTS: CRVO induced a rapid transient upregulation of Vegfa, a downregulation of Vegfb, and a delayed upregulation of Pedf in the neuroretina. In the neuroretina and retinal pigment epithelium, CRVO induced strong, transient downregulation of Kir4.1, Aqp4, and Aqp1, and striking rapid upregulation of Il1ss and Il6. Intravitreal triamcinolone reversed the downregulation of Kir4.1 and accelerated the normalization of the upregulated expression of Il1ss and Il6. The CRVO-induced transient upregulation of Vegfa was not influenced by the triamcinolone application. CONCLUSIONS: Triamcinolone exerts anti-inflammatory effects in the ischemic retina by inhibitory effects on the gene expression of IL-1ss and IL-6, and may have neuroprotective effects via improvement of retinal potassium homeostasis.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Aqp1  (aquaporin 1)
Aqp4  (aquaporin 4)
Il1b  (interleukin 1 beta)
Il6  (interleukin 6)
Kcnj10  (potassium inwardly-rectifying channel, subfamily J, member 10)
Serpinf1  (serpin family F member 1)
Vegfa  (vascular endothelial growth factor A)
Vegfb  (vascular endothelial growth factor B)

Genes (Mus musculus)
Aqp1  (aquaporin 1)
Aqp4  (aquaporin 4)
Il1b  (interleukin 1 beta)
Il6  (interleukin 6)
Kcnj10  (potassium inwardly-rectifying channel, subfamily J, member 10)
Serpinf1  (serine (or cysteine) peptidase inhibitor, clade F, member 1)
Vegfa  (vascular endothelial growth factor A)
Vegfb  (vascular endothelial growth factor B)

Genes (Homo sapiens)
AQP1  (aquaporin 1 (Colton blood group))
AQP4  (aquaporin 4)
IL1B  (interleukin 1 beta)
IL6  (interleukin 6)
KCNJ10  (potassium inwardly rectifying channel subfamily J member 10)
SERPINF1  (serpin family F member 1)
VEGFA  (vascular endothelial growth factor A)
VEGFB  (vascular endothelial growth factor B)


Additional Information