RGD Reference Report - Identification of TNF-related apoptosis-inducing ligand and other molecules that distinguish inflammatory from resident dendritic cells in patients with psoriasis. - Rat Genome Database

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Identification of TNF-related apoptosis-inducing ligand and other molecules that distinguish inflammatory from resident dendritic cells in patients with psoriasis.

Authors: Zaba, LC  Fuentes-Duculan, J  Eungdamrong, NJ  Johnson-Huang, LM  Nograles, KE  White, TR  Pierson, KC  Lentini, T  Suarez-Farinas, M  Lowes, MA  Krueger, JG 
Citation: Zaba LC, etal., J Allergy Clin Immunol. 2010 Jun;125(6):1261-1268.e9. Epub 2010 May 14.
RGD ID: 5147919
Pubmed: PMID:20471070   (View Abstract at PubMed)
PMCID: PMC2910451   (View Article at PubMed Central)
DOI: DOI:10.1016/j.jaci.2010.03.018   (Journal Full-text)

BACKGROUND: Previous work has identified CD11c(+)CD1c(-) dendritic cells (DCs) as the major "inflammatory" dermal DC population in patients with psoriasis vulgaris and CD1c(+) DCs as the "resident" cutaneous DC population. OBJECTIVE: We sought to further define molecular differences between these 2 myeloid dermal DC populations. METHODS: Inflammatory and resident DCs were single-cell sorted from lesional skin biopsy specimens of patients with psoriasis, and the transcriptome of CD11c(+)CD1c(-) versus CD1c(+) DCs was determined. Results were confirmed with RT-PCR, flow cytometry, immunohistochemistry, and double-labeled immunofluorescence. Human keratinocytes were cultured for functional studies. RESULTS: TNF-related apoptosis-inducing ligand (TRAIL), Toll-like receptors 1 and 2, S100A12/ENRAGE, CD32, and many other inflammatory products were differentially expressed in inflammatory DCs compared with resident DCs. Flow cytometry and immunofluorescence confirmed higher protein expression on CD1c(-) versus CD1c(+) DCs. TRAIL receptors, death receptor 4, and decoy receptor 2 were expressed in keratinocytes and dermal cells. In vitro culture of keratinocytes with TRAIL induced CCL20 chemokine. CONCLUSIONS: CD11c(+)CD1c(-) inflammatory DCs in psoriatic lesional skin express a wide range of inflammatory molecules compared with skin-resident CD1c(+) DCs. Some molecules made by inflammatory DCs, including TRAIL, could have direct effects on keratinocytes or other skin cell types to promote disease pathogenesis.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FCGR2AHumanpsoriasis  IEP mRNA:increased expression:dermal dendritic cellRGD 
Fcgr2aRatpsoriasis  ISOFCGR2A (Homo sapiens)mRNA:increased expression:dermal dendritic cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Fcgr2a  (Fc gamma receptor 2A)

Genes (Homo sapiens)
FCGR2A  (Fc gamma receptor IIa)


Additional Information