RGD Reference Report - Solution structure of a mammalian PCB-binding protein in complex with a PCB. - Rat Genome Database

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Solution structure of a mammalian PCB-binding protein in complex with a PCB.

Authors: Hard, T  Barnes, HJ  Larsson, C  Gustafsson, JA  Lund, J 
Citation: Hard T, etal., Nat Struct Biol. 1995 Nov;2(11):983-9.
RGD ID: 5144064
Pubmed: (View Article at PubMed) PMID:7583672

Metabolites of polychlorinated biphenyls (PCBs) bind with high affinity to uteroglobin, a small homodimeric protein that also binds progesterone. We present the solution structure of the reduced form of rat uteroglobin in complex with a PCB methylsulphone, (MeSO2)2-TCB. The structure reveals the molecular basis for the accumulation of (MeSO2)2-TCB by uteroglobin. The structure also shows how ligand binding and release might be controlled by reduction/oxidation of two intermolecular disulphide bonds. Breakage of these bonds induces a local unfolding of the N- and C-termini and a separation of helices creating a channel into the binding site. These effects make the ligand binding cavity readily accessible to entry of the ligand.

Annotation

Gene Ontology Annotations    

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Scgb1a1  (secretoglobin family 1A member 1)


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