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Chemokine-directed migration of tumor-inhibitory neural progenitor cells towards an intracranially growing glioma.

Authors: Honeth, G  Staflin, K  Kalliomaki, S  Lindvall, M  Kjellman, C 
Citation: Honeth G, etal., Exp Cell Res. 2006 May 1;312(8):1265-76. Epub 2006 Jan 23.
Pubmed: (View Article at PubMed) PMID:16434036
DOI: Full-text: DOI:10.1016/j.yexcr.2005.12.018

We have earlier shown that the rat neural progenitor cell line HiB5 is capable of suppressing intracranial growth of glioma cells in Fisher rats. Unlike some neural progenitor cells, HiB5 cells have not shown homing capacity towards glioma cells growing intracranially. In this study, we have genetically modified HiB5 progenitor cells to over-express the chemokine receptor CXCR3. We show that the introduced receptor is functionally responding to ligand stimulation with increased phosphorylation levels of ERK and SAPK/JNK and a transcriptional response of an AP-1 reporter system introduced into HIB5 cells. These transfected progenitor cells migrate in vitro in response to IP-10 and I-TAC. Further, we show an enhanced in vivo migration of the CXCR3 transfected HiB5 cells over the corpus callosum towards an IP-10 and I-TAC expressing glioma, as compared to wild type HiB5 cells. Our data indicate that it is possible to take advantage of chemokines natural capacity to initiate migratory responses, and to use this ability to enhance tumor-inhibitory neural progenitor cells to target an intracranially growing glioma.


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RGD Object Information
RGD ID: 5135509
Created: 2011-07-25
Species: All species
Last Modified: 2011-07-25
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.