RGD Reference Report - Human airway epithelial cells produce IP-10 (CXCL10) in vitro and in vivo upon rhinovirus infection. - Rat Genome Database

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Human airway epithelial cells produce IP-10 (CXCL10) in vitro and in vivo upon rhinovirus infection.

Authors: Spurrell, JC  Wiehler, S  Zaheer, RS  Sanders, SP  Proud, D 
Citation: Spurrell JC, etal., Am J Physiol Lung Cell Mol Physiol. 2005 Jul;289(1):L85-95. Epub 2005 Mar 11.
RGD ID: 5135489
Pubmed: PMID:15764644   (View Abstract at PubMed)
DOI: DOI:10.1152/ajplung.00397.2004   (Journal Full-text)

Human rhinovirus (HRV) infections trigger exacerbations of asthma and chronic obstructive pulmonary disease (COPD) and are associated with lymphocytic infiltration of the airways. We demonstrate that infection of primary cultures of human airway epithelial cells, or of the BEAS-2B human bronchial epithelial cell line, with human rhinovirus type 16 (HRV-16) induces expression of CXCL10 [IFN-gamma-inducible protein 10 (IP-10)], a ligand for the CXCR3 receptor found on activated type 1 T lymphocytes and natural killer cells. IP-10 mRNA reached maximal levels 24 h after HRV-16 infection then declined, whereas protein levels peaked 48 h after infection with no subsequent new synthesis. Cytosolic levels of AU-rich factor 1, a protein associated with mRNA destabilization, increased beginning 24 h after HRV-16 infection. Generation of IP-10 required virus capable of replication but was not dependent on prior induction of type 1 interferons. Transfection of synthetic double-stranded RNA into epithelial cells induced robust production of IP-10, whereas transfection of single-stranded RNA had no effect. Induction of IP-10 gene expression by HRV-16 depended upon activation of NF-kappaB, as well as other transcription factor recognition sequences further upstream in the IP-10 promoter. In vivo infection of human volunteers with HRV-16 strikingly increased IP-10 protein in nasal lavages during symptomatic colds. Levels of IP-10 correlated with symptom severity, viral titer, and numbers of lymphocytes in airway secretions. Thus IP-10 may play a role in the pathogenesis of HRV-induced colds and in HRV-induced exacerbations of COPD and asthma.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
common cold  IEP 5135489protein:increased expression:respiratory system fluid/secretionRGD 
common cold  ISOCXCL10 (Homo sapiens)5135489; 5135489protein:increased expression:respiratory system fluid/secretionRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl10  (C-X-C motif chemokine ligand 10)

Genes (Mus musculus)
Cxcl10  (C-X-C motif chemokine ligand 10)

Genes (Homo sapiens)
CXCL10  (C-X-C motif chemokine ligand 10)


Additional Information