RGD Reference Report - Role of neutrophils and NADPH phagocyte oxidase in host defense against respiratory infection with virulent Francisella tularensis in mice. - Rat Genome Database

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Role of neutrophils and NADPH phagocyte oxidase in host defense against respiratory infection with virulent Francisella tularensis in mice.

Authors: KuoLee, R  Harris, G  Conlan, JW  Chen, W 
Citation: KuoLee R, etal., Microbes Infect. 2011 May;13(5):447-56. Epub 2011 Jan 26.
RGD ID: 5135025
Pubmed: PMID:21277990   (View Abstract at PubMed)
DOI: DOI:10.1016/j.micinf.2011.01.010   (Journal Full-text)

Francisella tularensis subspecies (subsp.) tularensis is a CDC Category A biological warfare agent and inhalation of as few as 15 bacilli can initiate severe disease. Relatively little is known about the cellular and molecular mechanisms of host defense against respiratory infection with subsp. tularensis. In this study, we examined the role of neutrophils and NADPH phagocyte oxidase in host resistance to pulmonary infection in a mouse intranasal infection model. We found that despite neutrophil recruitment to the lungs and increased concentrations of neutrophil-chemotactic chemokines (KC, MIP-2 and RANTES) in the bronchoalveolar lavage fluid following intranasal inoculation of the pathogen, neither depletion of neutrophils nor enhancement of their recruitment into the lungs had any impact on bacterial burdens or survival rate/time. Nevertheless, mice deficient in NADPH phagocyte oxidase (gp91(phox/)) did exhibit higher tissue and blood bacterial burdens and succumbed to infection one day earlier than wild-type C57BL/6 mice. These results imply that although neutrophils are not a major effector cell in defense against subsp. tularensis infection, NADPH phagocyte oxidase does play a marginal role.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CXCL2Humantularemia  ISOCxcl2 (Mus musculus)protein:increased expression:respiratory system fluid/secretionRGD 
Cxcl2Rattularemia  ISOCxcl2 (Mus musculus)protein:increased expression:respiratory system fluid/secretionRGD 
Cxcl2Mousetularemia  IEP protein:increased expression:respiratory system fluid/secretionRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl2  (C-X-C motif chemokine ligand 2)

Genes (Mus musculus)
Cxcl2  (C-X-C motif chemokine ligand 2)

Genes (Homo sapiens)
CXCL2  (C-X-C motif chemokine ligand 2)


Additional Information