RGD Reference Report - Dependence on the motif YIPP for the physical association of Jak2 kinase with the intracellular carboxyl tail of the angiotensin II AT1 receptor. - Rat Genome Database

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Dependence on the motif YIPP for the physical association of Jak2 kinase with the intracellular carboxyl tail of the angiotensin II AT1 receptor.

Authors: Ali, MS  Sayeski, PP  Dirksen, LB  Hayzer, DJ  Marrero, MB  Bernstein, KE 
Citation: Ali MS, etal., J Biol Chem. 1997 Sep 12;272(37):23382-8.
RGD ID: 5133683
Pubmed: PMID:9287353   (View Abstract at PubMed)

Angiotensin II is the effector molecule of the renin-angiotensin system. Virtually all of its biochemical actions are mediated through a single class of cell-surface receptors called AT1. These receptors contain the structural features of the seven-transmembrane, G-protein-coupled receptor superfamily. Angiotensin II, acting through the AT1 receptor, also stimulates the Jak/STAT pathway by inducing ligand-dependent Jak2 tyrosine phosphorylation and activation. Here, we show that a glutathione S-transferase fusion protein containing the carboxyl-terminal 54 amino acids of the rat AT1A receptor physically binds to Jak2 in an angiotensin II-dependent manner. Deletional analysis, using both in vitro protocols and cell transfection analysis, showed that this association is dependent on the AT1A receptor motif YIPP (amino acids 319-322). The wild-type AT1A receptor can induce Jak2 tyrosine phosphorylation. In contrast, an AT1A receptor lacking the YIPP motif is unable to induce ligand-dependent phosphorylation of Jak2. Competition experiments with synthetic peptides suggest that each of the YIPP amino acids, including tyrosine 319, is important in Jak2 binding to the AT1A receptor. The binding of the AT1A receptor to the intracellular tyrosine kinase Jak2 supports the concept that the seven-transmembrane superfamily of receptors can physically associate with enzymatically active intracellular proteins, creating a signaling complex mechanistically similar to that observed with growth factor and cytokine receptors.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Agtr1aRatprotein kinase binding  IPIJak2 (Rattus norvegicus) RGD 
Jak2Rattype 1 angiotensin receptor binding  IPIAgtr1a (Rattus norvegicus)protein C-terminus bindingRGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Agtr1a  (angiotensin II receptor, type 1a)
Jak2  (Janus kinase 2)

Genes (Mus musculus)
Agtr1a  (angiotensin II receptor, type 1a)
Jak2  (Janus kinase 2)

Genes (Homo sapiens)
AGTR1  (angiotensin II receptor type 1)
JAK2  (Janus kinase 2)


Additional Information