RGD Reference Report - Proteome analysis of human pancreatic ductal adenocarcinoma tissue using two-dimensional gel electrophoresis and tandem mass spectrometry for identification of disease-related proteins. - Rat Genome Database

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Proteome analysis of human pancreatic ductal adenocarcinoma tissue using two-dimensional gel electrophoresis and tandem mass spectrometry for identification of disease-related proteins.

Authors: Tian, R  Wei, LM  Qin, RY  Li, Y  Du, ZY  Xia, W  Shi, CJ  Jin, H 
Citation: Tian R, etal., Dig Dis Sci. 2008 Jan;53(1):65-72. Epub 2007 May 11.
RGD ID: 5132867
Pubmed: PMID:17492507   (View Abstract at PubMed)
DOI: DOI:10.1007/s10620-007-9823-3   (Journal Full-text)

A comparative proteomic approach has been used to identify and analyze proteins related to pancreatic cancer. Proteomes of eight pairs of clinical pancreatic ductal adenocarcinoma (PDAC) tissue samples and samples of normal adjacent tissue were obtained by two-dimensional gel electrophoresis (2DE). Comprehensive analysis of proteins was focused on total protein spots for which there were statistical differences between the two groups. Proteins were identified by peptide mass fingerprinting with tandem mass spectrometry (MS-MS). Western blotting and immunohistochemistry (IHC) were also performed to verify the expression of some candidate proteins. Thirty protein spots were identified, including proteases, antioxidant proteins, signal-transduction proteins, calcium-binding proteins, structural proteins, chaperones, and others. Western blotting and IHC confirmed up-regulated expression of two candidate proteins, nucleotide diphosphatase kinase (NDPK) and annexin II, in tumorous tissues. These results suggest that combination of 2DE with MS is an effective strategy for discovery of differently expressed proteins in PDAC which may be molecular markers for diagnosis or therapeutic targets.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
pancreatic ductal carcinoma  IEP 5132867protein:increased expression:pancreas (human)RGD 
pancreatic ductal carcinoma  ISONME1 (Homo sapiens)5132867; 5132867protein:increased expression:pancreas (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nme1  (NME/NM23 nucleoside diphosphate kinase 1)

Genes (Mus musculus)
Nme1  (NME/NM23 nucleoside diphosphate kinase 1)

Genes (Homo sapiens)
NME1  (NME/NM23 nucleoside diphosphate kinase 1)


Additional Information