RGD Reference Report - Integrin alpha(5) interacts with EGFR, is necessary for FcvarepsilonRI signaling and is necessary for allergic inflammation in relation with angiogenesis. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Integrin alpha(5) interacts with EGFR, is necessary for FcvarepsilonRI signaling and is necessary for allergic inflammation in relation with angiogenesis.

Authors: Kim, Y  Kim, K  Park, D  Eom, S  Park, H  Lee, H  Lee, YS  Choe, J  Hahn, JH  Kim, YM  Ro, JY  Jeoung, D 
Citation: Kim Y, etal., Mol Immunol. 2011 Apr;48(8):1035-45. Epub 2011 Feb 23.
RGD ID: 5131469
Pubmed: PMID:21349584   (View Abstract at PubMed)
DOI: DOI:10.1016/j.molimm.2011.01.013   (Journal Full-text)

Recent reports have suggested role for epidermal growth factor receptor (EGFR) in asthma and skin inflammation. Integrin(s) are known to be necessary for the transactivation of EGFR. The roles of EGFR and integrin(s) in allergic inflammation were investigated. Antigen stimulation induced activation of EGFR and interaction between EGFR and integrin alpha(5) in Rat Basophilic Leukemia (RBL2H3) cells and bone marrow-derived mouse mast cells (BMMCs). Flow cytometry revealed increased phosphorylation of EGFR on cell surfaces. Antigen stimulation induced interaction between EGFR and FcvarepsilonRI in both RBL2H3 cells and BMMCs. Blocking of EGFR or integrin alpha exerted negative effects on rac1 activity and secretion of beta-hexosaminidase in both RBL2H3 cells and BMMCs. EGFR and integrin alpha(5) were found to be necessary for IgE-dependent cutaneous anaphylaxis. FAK (focal adhesion kinase), interacted with EGFR and with FcvarepsilonRI upon antigen stimulation, and it was necessary for the increased secretion of beta-hexosaminidase in both RBL2H3 cells and BMMCs. EGFR and integrin alpha(5) were necessary for interactions between activated RBL2H3 cells, BMMCs and rat aortic endothelial cells (RAECs). Conditioned medium of antigen-stimulated RBL2H3 cells promoted RAECs tube formation, rat aortic ring formation and blood vessel formation. Conditioned medium of antigen-stimulated BMMCs also had the same effects on RAECs. This enhanced angiogenic potential of RAECs was dependent on EGFR and integrin alpha(5). In conclusion, EGFR, via interaction with FcvarepsilonRI and integrin alpha(5), is necessary for allergic inflammation associated with cellular interaction.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell surface  IDA 5131469 RGD 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Cd44  (CD44 molecule)
Egfr  (epidermal growth factor receptor)
Itga5  (integrin subunit alpha 5)
Ms4a1  (membrane spanning 4-domains A1)


Additional Information