RGD Reference Report - Interaction of 5-hydroxytryptamine and tumor necrosis factor-alpha to pain-related behavior by nucleus pulposus applied on the nerve root in rats. - Rat Genome Database

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Interaction of 5-hydroxytryptamine and tumor necrosis factor-alpha to pain-related behavior by nucleus pulposus applied on the nerve root in rats.

Authors: Kobayashi, H  Kikuchi, S  Konno, S  Kato, K  Sekiguchi, M 
Citation: Kobayashi H, etal., Spine (Phila Pa 1976). 2011 Feb 1;36(3):210-8.
RGD ID: 5130897
Pubmed: PMID:21248590   (View Abstract at PubMed)
DOI: DOI:10.1097/BRS.0b013e3181fea618   (Journal Full-text)

STUDY DESIGN: The effects of exogenous 5-hydroxytryptamine (5-HT), tumor necrosis factor(TNF)-alpha, 5-HT + TNF in combination, and autologous nucleus pulposus (NP) at dorsal root ganglion (DRG) were examined using rat models. OBJECTIVE: To examine the interaction of 5-HT with TNF for pain-related behavior in a rat lumbar disc herniation (LDH) model. SUMMARY OF BACKGROUND DATA: 5-HT and TNF have been shown to play roles in sciatica in lumbar disc herniation as chemical factors. METHODS: Adult female Sprague-Dawley rats were divided into six groups: 5-HT group, TNF group, 5-HT + TNF (combination) group, NP group, control group, and naive group. Von Frey tests were used for pain-related behavior testing. Expressions of activating transcription factor 3 and calcitonin gene-related peptide (CGRP) were evaluated immunohistochemically. Expressions of TNF, TNF receptor 1 (TNFR1), and 5-HT2A receptors in the left L5 DRG were examined using western blotting. Plasma levels of 5-hydroxyindole acetic acid, a metabolite of 5-HT, were measured. RESULTS: Mechanical withdrawal thresholds were significantly decreased in the 5-HT, TNF, combination, and NP groups compared with controls. Thresholds recovered after 14 days in the 5-HT and TNF groups, and after 28 days in the combination group. Exogenous 5-HT and TNF to the nerve root induced pain-related behavior and lasted for a shorter period compared with combination and NP groups. Activating transcription factor 3- and calcitonin gene-related peptide-immunoreactive DRG neurons were significantly increased only in the early phase in 5-HT, TNF, combination, and NP groups. TNF induced 5-HT2A receptor expressions in the DRG, while 5-HT induced TNF and TNF receptor 1 expressions. CONCLUSION: The present findings suggest that both 5-HT and TNF induce pain-related behavior and interact with each other to prolong pain-related behavior in a rat LDH model.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Pain  ISOTnfrsf1a (Rattus norvegicus)5130897; 5130897protein:increased expression:dorsal root ganglion (rat)RGD 
Pain  IEP 5130897protein:increased expression:dorsal root ganglion (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnfrsf1a  (TNF receptor superfamily member 1A)

Genes (Mus musculus)
Tnfrsf1a  (tumor necrosis factor receptor superfamily, member 1a)

Genes (Homo sapiens)
TNFRSF1A  (TNF receptor superfamily member 1A)


Additional Information