RGD Reference Report - Unilateral ureteral obstruction evokes renal tubular apoptosis via the enhanced oxidative stress and endoplasmic reticulum stress in the rat. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Unilateral ureteral obstruction evokes renal tubular apoptosis via the enhanced oxidative stress and endoplasmic reticulum stress in the rat.

Authors: Yeh, CH  Chiang, HS  Lai, TY  Chien, CT 
Citation: Yeh CH, etal., Neurourol Urodyn. 2011 Mar;30(3):472-9. doi: 10.1002/nau.20855. Epub 2011 Feb 8.
RGD ID: 5130876
Pubmed: PMID:21305585   (View Abstract at PubMed)
DOI: DOI:10.1002/nau.20855   (Journal Full-text)

PURPOSE: Oxidative stress and endoplasmic reticulum (ER) stress may induce renal apoptosis and contribute to the pathogenesis of the kidney with unilateral ureteral obstruction (UUO). MATERIALS AND METHODS: We induced UUO the female Wistar rats by ligation of the left ureter at the ureteropelvic junction. The UUO kidney was performed from 4 hr to 7 days course. At the indicated time, we measured the arterial blood pressure and renal blood flow in each rat, renal ROS measurement in vivo by a chemiluminescence analyzer. We performed immunohistochemistry of monocyte/macrophage (ED-1) stain for leukocyte infiltration, 4-hydroxynoneal (4-HNE) stain for ROS products, and apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) and Western blot to analyze ER stress-associated and apoptosis-related proteins expression in the UUO kidney. RESULTS: We found that UUO decreased renal blood flow and increased renal vascular resistance and renal ROS. UUO decreased renal manganese superoxide dismutase (MnSOD) and catalase protein expression in a time-dependent manner. Increased 4-HNE stain in the renal tubules and ED-1 stain in the renal tubulointerstitial compartment occurred after 4 hr of UUO in the kidney. UUO significantly enhanced ER stress markers like ER stress-response protein 25 and glucose-regulated protein 78 and ER-associated apoptosis proteins, c-JUN NH(2) -terminal kinase, and caspase 12, in the kidney. Subsequently, UUO enhanced renal pro-apoptotic Bax and caspase 3 expression and decreased anti-apoptotic Bcl-2 expression, leading to renal tubular apoptosis. CONCLUSIONS: Our data suggest that renal tubular apoptosis induced by oxidative stress and ER stress occurred in the UUO kidney. Neurourol. Urodynam. 30:472-479, 2011. (c) 2011 Wiley-Liss, Inc.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ureteral obstruction  ISOCat (Rattus norvegicus)5130876; 5130876protein:decreased expression:kidneyRGD 
ureteral obstruction  IEP 5130876protein:decreased expression:kidneyRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cat  (catalase)

Genes (Mus musculus)
Cat  (catalase)

Genes (Homo sapiens)
CAT  (catalase)


Additional Information