RGD Reference Report - High MMP-9 activity characterises pleural tuberculosis correlating with granuloma formation. - Rat Genome Database

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High MMP-9 activity characterises pleural tuberculosis correlating with granuloma formation.

Authors: Sheen, P  O'Kane, CM  Chaudhary, K  Tovar, M  Santillan, C  Sosa, J  Caviedes, L  Gilman, RH  Stamp, G  Friedland, JS 
Citation: Sheen P, etal., Eur Respir J. 2009 Jan;33(1):134-41. Epub 2008 Aug 20.
RGD ID: 5129700
Pubmed: PMID:18715875   (View Abstract at PubMed)
DOI: DOI:10.1183/09031936.00127807   (Journal Full-text)

Tuberculosis (TB) pleural disease is complicated by extensive tissue destruction. Matrix metalloproteinase (MMP)-1 and -9 are implicated in immunopathology of pulmonary and central nervous system TB. There are few data on MMP activity in TB pleurisy. The present study investigated MMP-1, -2 and -9 and their specific inhibitors (tissue inhibitor of metalloproteinase (TIMP)-1 and -2) in tuberculous effusions, and correlated these with clinical and histopathological features. Clinical data, routine blood tests, and pleural fluid/biopsy material were obtained from 89 patients presenting with pleural effusions in a TB-endemic area. MMP-1, -2 and -9 were measured by zymography or western blot, and TIMP-1 and -2 by ELISA. Pleural biopsies were examined microscopically, cultured for acid-alcohol fast bacilli and immunostained for MMP-9. Tuberculous pleural effusions contained the highest concentrations of MMP-9 compared with malignant effusions or heart failure transudates. MMP-9 concentrations were highest in effusions from patients with granulomatous biopsies: median (interquartile range) 108 (61-218) pg x mL(-1) versus 43 (12-83) pg x mL(-1) in those with nongranulomatous pleural biopsies. MMP-1 and -2 were not upregulated in tuberculous pleural fluid. The ratio of MMP-9:TIMP-1 was significantly higher in TB effusions. Tuberculous pleurisy is characterised by a specific pattern of matrix metalloproteinase-9 upregulation, correlating with the presence of granulomas and suggesting a specific role for matrix metalloproteinase-9 in inflammatory responses in tuberculous pleural disease.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
pleural tuberculosis  IEP 5129700protein:increased expression:pleura:RGD 
pleural tuberculosis  ISOMMP9 (Homo sapiens)5129700; 5129700protein:increased expression:pleura:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mmp9  (matrix metallopeptidase 9)

Genes (Mus musculus)
Mmp9  (matrix metallopeptidase 9)

Genes (Homo sapiens)
MMP9  (matrix metallopeptidase 9)


Additional Information