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Gene expression microarray analysis of early oxygen-induced retinopathy in the rat.

Authors: Tea, M  Fogarty, R  Brereton, HM  Michael, MZ  Van der Hoek, MB  Tsykin, A  Coster, DJ  Williams, KA 
Citation: Tea M, etal., J Ocul Biol Dis Infor. 2009 Dec 12;2(4):190-201.
Pubmed: (View Article at PubMed) PMID:20157446
DOI: Full-text: DOI:10.1007/s12177-009-9041-7

Different inbred strains of rat differ in their susceptibility to oxygen-induced retinopathy (OIR), an animal model of human retinopathy of prematurity. We examined gene expression in Sprague-Dawley (susceptible) and Fischer 344 (resistant) neonatal rats after 3 days exposure to cyclic hyperoxia or room air, using Affymetrix rat Genearrays. False discovery rate analysis was used to identify differentially regulated genes. Such genes were then ranked by fold change and submitted to the online database, DAVID. The Sprague-Dawley list returned the term "response to hypoxia," absent from the Fischer 344 output. Manual analysis indicated that many genes known to be upregulated by hypoxia-inducible factor-1alpha were downregulated by cyclic hyperoxia. Quantitative real-time RT-PCR analysis of Egln3, Bnip3, Slc16a3, and Hk2 confirmed the microarray results. We conclude that combined methodologies are required for adequate dissection of the pathophysiology of strain susceptibility to OIR in the rat. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12177-009-9041-7) contains supplementary material, which is available to authorized users.


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RGD Object Information
RGD ID: 5128796
Created: 2011-03-16
Species: All species
Last Modified: 2011-03-16
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.