RGD Reference Report - [Effects of decreased leptin expression on liver fibrosis]. - Rat Genome Database

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[Effects of decreased leptin expression on liver fibrosis].

Authors: Feng, Hai-juan  Zhu, Jing  Pan, Liang  Lu, Jing-xian  Xiao, Ming-bing  Huang, Hua  Ni, Run-zhou  Lu, Cui-hua 
Citation: Feng HJ, etal., Zhonghua Gan Zang Bing Za Zhi. 2010 May;18(5):342-5. doi: 10.3760/cma.j.issn.1007-3418.2010.05.008.
RGD ID: 5128589
Pubmed: PMID:20509998   (View Abstract at PubMed)


OBJECTIVE: To study the effects of decreased leptin expression on liver fibrosis.
METHODS: The small interfering RNA, targeting leptin gene, was designed according to the secondary structure of leptin gene. The recombinant plasmids were encapsulated with lipofectamine and then injected into carbon tetrachloride (CCl4) induced rat liver fibrosis models. Leptin and I, III collage were detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR).
RESULTS: The mRNA and protein levels of leptin in the fibrotic liver transfected with leptin shRNA were significantly decreased compared with those in controls (P less than 0.01). The depositions of type I and type III collagens were also decreased (P less than 0.01).
CONCLUSION: Decreased leptin expression prevents liver fibrosis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Liver Cirrhosis  ISOLep (Rattus norvegicus)5128589; 5128589 RGD 
Experimental Liver Cirrhosis  IMP 5128589 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lep  (leptin)

Genes (Mus musculus)
Lep  (leptin)

Genes (Homo sapiens)
LEP  (leptin)


Additional Information