RGD Reference Report - Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events. - Rat Genome Database

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Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events.

Authors: Pryor, PR  Mullock, BM  Bright, NA  Lindsay, MR  Gray, SR  Richardson, SC  Stewart, A  James, DE  Piper, RC  Luzio, JP 
Citation: Pryor PR, etal., EMBO Rep. 2004 Jun;5(6):590-5. Epub 2004 May 7.
RGD ID: 4892614
Pubmed: PMID:15133481   (View Abstract at PubMed)
PMCID: PMC1299070   (View Article at PubMed Central)
DOI: DOI:10.1038/sj.embor.7400150   (Journal Full-text)

Both heterotypic and homotypic fusion events are required to deliver endocytosed macromolecules to lysosomes and remodel late endocytic organelles. A trans-SNARE complex consisting of Q-SNAREs syntaxin 7, Vti1b and syntaxin 8 and the R-SNARE VAMP8 has been shown by others to be responsible for homotypic fusion of late endosomes. Using antibody inhibition experiments in rat liver cell-free systems, we confirmed this result, but found that the same Q-SNAREs can combine with an alternative R-SNARE, namely VAMP7, for heterotypic fusion between late endosomes and lysosomes. Co-immunoprecipitation demonstrated separate syntaxin 7 complexes with either VAMP7 or VAMP8 in solubilized rat liver membranes. Additionally, overexpression of the N-terminal domain of VAMP7, in cultured fibroblastic cells, inhibited the mixing of a preloaded lysosomal content marker with a marker delivered to late endosomes. These data show that combinatorial interactions of SNAREs determine whether late endosomes undergo homotypic or heterotypic fusion events.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Stx7Ratlate endosome membrane  IDA  RGD 
Stx8Ratlate endosome membrane  IDA  RGD 
Vamp7Ratlate endosome membrane  IDA  RGD 
Vamp8Ratlate endosome membrane  IDA  RGD 
Stx7Ratlysosomal membrane  IDA  RGD 
Stx8Ratlysosomal membrane  IDA  RGD 
Vamp7Ratlysosomal membrane  IDA  RGD 
Vamp8Ratlysosomal membrane  IDA  RGD 
Stx7RatSNARE complex part_ofIDA PMID:15133481UniProt 
Stx8RatSNARE complex part_ofIDA PMID:15133481UniProt 
Vamp7RatSNARE complex part_ofIDA PMID:15133481UniProt 
Vti1bRatSNARE complex part_ofIDA PMID:15133481UniProt 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Stx7  (syntaxin 7)
Stx8  (syntaxin 8)
Vamp7  (vesicle-associated membrane protein 7)
Vamp8  (vesicle-associated membrane protein 8)
Vti1b  (vesicle transport through interaction with t-SNAREs 1B)


Additional Information