RGD Reference Report - In vivo role of dendritic cells in a murine model of pulmonary cryptococcosis. - Rat Genome Database

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In vivo role of dendritic cells in a murine model of pulmonary cryptococcosis.

Authors: Wozniak, KL  Vyas, JM  Levitz, SM 
Citation: Wozniak KL, etal., Infect Immun. 2006 Jul;74(7):3817-24.
RGD ID: 4892554
Pubmed: (View Article at PubMed) PMID:16790753
DOI: Full-text: DOI:10.1128/IAI.00317-06

Dendritic cells (DC) have been shown to phagocytose and kill Cryptococcus neoformans in vitro and are believed to be important for inducing protective immunity against this organism. Exposure to C. neoformans occurs mainly by inhalation, and in this study we examined the in vivo interactions of C. neoformans with DC in the lung. Fluorescently labeled live C. neoformans and heat-killed C. neoformans were administered intranasally to C57BL/6 mice. At specific times postinoculation, mice were sacrificed, and lungs were removed. Single-cell suspensions of lung cells were prepared, stained, and analyzed by microscopy and flow cytometry. Within 2 h postinoculation, fluorescently labeled C. neoformans had been internalized by DC, macrophages, and neutrophils in the mouse lung. Additionally, lung DC from mice infected for 7 days showed increased expression of the maturation markers CD80, CD86, and major histocompatibility complex class II. Finally, ex vivo incubation of lung DC from infected mice with Cryptococcus-specific T cells resulted in increased interleukin-2 production compared to the production by DC from naive mice, suggesting that there was antigen-specific T-cell activation. This study demonstrated that DC in the lung are capable of phagocytosing Cryptococcus in vivo and presenting antigen to C. neoformans-specific T cells ex vivo, suggesting that these cells have roles in innate and adaptive pulmonary defenses against cryptococcosis.

Disease Annotations    
cryptococcosis  (IEP,ISO)
systemic scleroderma  (IAGP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Cd86  (CD86 molecule)

Genes (Mus musculus)
Cd86  (CD86 antigen)

Genes (Homo sapiens)
CD86  (CD86 molecule)

Additional Information