RGD Reference Report - Exhaled NO and iNOS expression in sputum cells of healthy, obese and OSA subjects. - Rat Genome Database

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Exhaled NO and iNOS expression in sputum cells of healthy, obese and OSA subjects.

Authors: Depalo, A  Carpagnano, GE  Spanevello, A  Sabato, R  Cagnazzo, MG  Gramiccioni, C  Foschino-Barbaro, MP 
Citation: Depalo A, etal., J Intern Med. 2008 Jan;263(1):70-8.
RGD ID: 4891935
Pubmed: PMID:18098375   (View Abstract at PubMed)

BACKGROUND: Obstructive sleep apnoea (OSA) is associated with airways inflammation; a key role in this regard seems to be played by nitric oxide (NO). The aim of this study was to measure exhaled NO and expression of its enzyme, the inducible nitric oxide synthase (iNOS) in cells of induced sputum in OSA patients and in obese subjects without sleep apnoea and to correlate these inflammatory markers with severity of OSA. METHODS: We enrolled 18 obese patients with OSA (10 men, age 48.2 +/- 8.4 years), 15 obese patients without OSA (eight men, age 52.8 +/- 11 years) and 10 healthy subjects (five men, age 42 +/- 4 years). Exhaled NO was measured using a chemiluminescence analyser; iNOS expression was measured in the sputum cells by immunocytochemistry. RESULTS: Exhaled NO resulted significantly increased in OSA and in obese patients (23.1 +/- 2.1 and 17.9 +/- 2.1 p.p.b.) than in healthy subjects (7.2 +/- 0.6 p.p.b.; P < 0.001). OSA and obese patients showed a higher percentage of neutrophils and a lower percentage of macrophages in the induced sputum compared to healthy subjects. In addition, OSA and obese patients showed higher iNOS expression in neutrophils and in macrophages with respect to healthy subjects. A positive correlation between exhaled NO, iNOS expression and AHI was observed. CONCLUSIONS: These data confirm the presence of airway inflammation in OSA and in obese patients, and suggest the possible role for NO and iNOS expression in neutrophils of the induced sputum as noninvasive markers to identify and monitor the airway inflammation in these subjects.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
obesity  IEP 4891935protein:increased expression:sputumRGD 
obesity  ISONOS2 (Homo sapiens)4891935; 4891935protein:increased expression:sputumRGD 
obstructive sleep apnea  IEP 4891935associated with obesity and protein:increased expression:sputumRGD 
obstructive sleep apnea  ISONOS2 (Homo sapiens)4891935; 4891935associated with obesity and protein:increased expression:sputumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Nos2  (nitric oxide synthase 2)

Genes (Mus musculus)
Nos2  (nitric oxide synthase 2, inducible)

Genes (Homo sapiens)
NOS2  (nitric oxide synthase 2)


Additional Information