RGD Reference Report - Involvement of fractalkine/CX3CL1 expression by dendritic cells in the enhancement of host immunity against Legionella pneumophila. - Rat Genome Database

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Involvement of fractalkine/CX3CL1 expression by dendritic cells in the enhancement of host immunity against Legionella pneumophila.

Authors: Kikuchi, T  Andarini, S  Xin, H  Gomi, K  Tokue, Y  Saijo, Y  Honjo, T  Watanabe, A  Nukiwa, T 
Citation: Kikuchi T, etal., Infect Immun. 2005 Sep;73(9):5350-7.
RGD ID: 4891897
Pubmed: PMID:16113250   (View Abstract at PubMed)
PMCID: PMC1231053   (View Article at PubMed Central)
DOI: DOI:10.1128/IAI.73.9.5350-5357.2005   (Journal Full-text)

Legionnaires' disease is clinically manifested as severe pneumonia caused by Legionella pneumophila. However, the dendritic cell (DC)-centered immunological framework of the host defense against L. pneumophila has not been fully delineated. For this study, we focused on a potent chemoattractant for lymphocytes, fractalkine/CX3CL1, and observed that the fractalkine expression of DCs was somewhat up-regulated when they encountered L. pneumophila. We therefore hypothesized that fractalkine expressed by Legionella-capturing DCs is involved in the induction of T-cell-mediated immune responses against Legionella, which would be enhanced by a genetic modulation of DCs to overexpress fractalkine. In vivo immunization-challenge experiments demonstrated that DCs modified with a recombinant adenovirus vector to overexpress fractalkine (AdFKN) and pulsed with heat-killed Legionella protected immunized mice from a lethal Legionella infection and that the generation of in vivo protective immunity depended on the host lymphocyte subsets, including CD4(+) T cells, CD8(+) T cells, and B cells. Consistent with this, immunization with AdFKN/Legionella/DC induced significantly higher levels of serum anti-Legionella antibodies of several isotypes than those induced by control immunizations. Further analysis of spleen cells from the immunized mice indicated that the AdFKN/Legionella/DC immunization elicited Th1-dominated immune responses to L. pneumophila. These observations suggest that fractalkine may play an important role in the DC-mediated host defense against intracellular pathogens such as L. pneumophila.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CX3CL1HumanLegionnaires' disease  ISOCx3cl1 (Mus musculus) RGD 
Cx3cl1RatLegionnaires' disease  ISOCx3cl1 (Mus musculus) RGD 
Cx3cl1MouseLegionnaires' disease  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cx3cl1  (C-X3-C motif chemokine ligand 1)

Genes (Mus musculus)
Cx3cl1  (C-X3-C motif chemokine ligand 1)

Genes (Homo sapiens)
CX3CL1  (C-X3-C motif chemokine ligand 1)


Additional Information