RGD Reference Report - Chemokines released from astrocytes promote chemokine receptor 5-mediated neuronal cell differentiation. - Rat Genome Database

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Chemokines released from astrocytes promote chemokine receptor 5-mediated neuronal cell differentiation.

Authors: Park, MH  Lee, YK  Lee, YH  Kim, YB  Yun, YW  Nam, SY  Hwang, SJ  Han, SB  Kim, SU  Hong, JT 
Citation: Park MH, etal., Exp Cell Res. 2009 Oct 1;315(16):2715-26. Epub 2009 Jun 24.
RGD ID: 4889986
Pubmed: PMID:19559698   (View Abstract at PubMed)
DOI: DOI:10.1016/j.yexcr.2009.06.017   (Journal Full-text)

Astrocytes are one of major glial cell types in the central nervous system (CNS), and can support many functions of neuronal cells. In the present study, we demonstrated that the differentiation of rat embryonic neuronal cells was promoted by treatment with astrocyte and microglia-conditioned medium. Cytokine assays identified that the IL-4, MIP-1, KC, and RANTES as were released from astrocyte, and these chemokines promote differentiation of rat embryonic neuronal cells. However, chemokine-promoted neuronal cell differentiation was suppressed by antibodies of these chemokines and their receptor (CCR5). CCR5 and neuronal cell differentiation marker proteins were found to be colocalized, and their expressions were enhanced by chemokines. Furthermore, the differentiation of neuronal cells from CCR5 knock-out mice and of neuronal cells from mice knocked down with the CCR5 siRNA were significantly reduced and delayed. Bradykinin elevated calcium influx in the embryonic neuronal cells. These data suggest that specific chemokines derived from astrocytes may significantly have influence on the CCR5-mediated differentiation of embryonic neuronal cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ccl5Ratpositive regulation of gene expression  IMP Ccr5, nestin, neunRGD 
Ccr5Ratpositive regulation of gene expression  IMP Nestin, NeunRGD 
Ccl5Ratpositive regulation of neuron differentiation  IMP  RGD 
Ccr5Ratpositive regulation of neuron differentiation  IMP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NesRatCCR5 chemokine receptor binding  IPICcr5 (Rattus norvegicus) RGD 
Ccr5Ratprotein binding  IPINes (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccl5  (C-C motif chemokine ligand 5)
Ccr5  (C-C motif chemokine receptor 5)
Nes  (nestin)

Objects referenced in this article
Gene CCR5 C-C motif chemokine receptor 5 Homo sapiens
Gene Ccr5 C-C motif chemokine receptor 5 Mus musculus

Additional Information