RGD Reference Report - Membrane localization, Caveolin-3 association and rapid actions of vitamin D receptor in cardiac myocytes. - Rat Genome Database

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Membrane localization, Caveolin-3 association and rapid actions of vitamin D receptor in cardiac myocytes.

Authors: Zhao, G  Simpson, RU 
Citation: Zhao G and Simpson RU, Steroids. 2010 Aug-Sep;75(8-9):555-9. Epub 2009 Dec 14.
RGD ID: 4889912
Pubmed: PMID:20015453   (View Abstract at PubMed)
PMCID: PMC2885558   (View Article at PubMed Central)
DOI: DOI:10.1016/j.steroids.2009.12.001   (Journal Full-text)

The active form of vitamin D, 1alpha, 25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), mediates both genomic and rapid non-genomic actions in heart cells. We have previously shown that the vitamin D receptor (VDR) is located in the t-tubular structure of cardiomyocytes. Here we show that VDR specifically interacts with Caveolin-3 in the t-tubules and sarcolemma of adult rat cardiac myocytes. Co-immunoprecipitation studies using VDR antibodies revealed that Caveolin-3 specifically co-precipitates with the VDR and similarly the VDR is co-precipitated with Caveolin-3 antibody. Confocal immuno-fluorescence microscopy analysis also showed co-localization of VDR and Caveolin-3 in t-tubules and sarcolemma. The non-genomic effects of the functional VDR were studied in electrically stimulated myocytes isolated from adult rat hearts. Sarcomere shortening and re-lengthening were measured in 1,25(OH)(2)D(3) treated cardiac myocytes. A 1nM treatment decreased peak shortening within minutes, suggesting a rapid effect through the membrane-bound VDR. This novel finding of the interaction between VDR and Caveolin-3 is fundamentally important in understanding 1,25(OH)(2)D(3) signal transduction in heart cells and provides further evidence that VDR plays a role in regulation of heart structure and function.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
T-tubule  IDA 4889912 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIVdr (Rattus norvegicus)4889912 RGD 
protein binding  IPICav3 (Rattus norvegicus)4889912 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cav3  (caveolin 3)
Vdr  (vitamin D receptor)


Additional Information