RGD Reference Report - Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes. - Rat Genome Database

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Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes.

Authors: Young, AR  Chan, EY  Hu, XW  Kochl, R  Crawshaw, SG  High, S  Hailey, DW  Lippincott-Schwartz, J  Tooze, SA 
Citation: Young AR, etal., J Cell Sci. 2006 Sep 15;119(Pt 18):3888-900. Epub 2006 Aug 29.
RGD ID: 4889884
Pubmed: PMID:16940348   (View Abstract at PubMed)
DOI: DOI:10.1242/jcs.03172   (Journal Full-text)

Autophagy, fundamentally a lysosomal degradation pathway, functions in cells during normal growth and certain pathological conditions, including starvation, to maintain homeostasis. Autophagosomes are formed through a mechanism that is not well understood, despite the identification of many genes required for autophagy. We have studied the mammalian homologue of Atg9p, a multi-spanning transmembrane protein essential in yeast for autophagy, to gain a better understanding of the function of this ubiquitious protein. We show that both the N- and C-termini of mammalian Atg9 (mAtg9) are cytosolic, and predict that mAtg9 spans the membrane six times. We find that mAtg9 is located in the trans-Golgi network and late endosomes and colocalizes with TGN46, the cation-independent mannose-6-phosphate receptor, Rab7 and Rab9. Amino acid starvation or rapamycin treatment, which upregulates autophagy, causes a redistribution of mAtg9 from the TGN to peripheral, endosomal membranes, which are positive for the autophagosomal marker GFP-LC3. siRNA-mediated depletion of the putative mammalian homologue of Atg1p, ULK1, inhibits this starvation-induced redistribution. The redistribution of mAtg9 also requires PI 3-kinase activity, and is reversed after restoration of amino acids. We speculate that starvation-induced autophagy, which requires mAtg9, may rely on an alteration of the steady-state trafficking of mAtg9, in a Atg1-dependent manner.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
late endosome  IDA 4889884 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Atg9a  (autophagy related 9A)


Additional Information