RGD Reference Report - A 1.8-Mbp fragment on chromosome 1 affects sympathetic response to stress: evaluation in reciprocal congenic strains between stroke-prone spontaneously hypertensive rat and Wistar-Kyoto rat. - Rat Genome Database

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A 1.8-Mbp fragment on chromosome 1 affects sympathetic response to stress: evaluation in reciprocal congenic strains between stroke-prone spontaneously hypertensive rat and Wistar-Kyoto rat.

Authors: Xiao, B  Harada, Y  Kawakami, K  Nabika, T 
Citation: Xiao B, etal., J Hypertens. 2010 Nov 8.
RGD ID: 4889147
Pubmed: PMID:21063213   (View Abstract at PubMed)
DOI: DOI:10.1097/HJH.0b013e32834137cd   (Journal Full-text)

BACKGROUND: In the previous studies, we indicated that a gene (or genes) responsible for exaggerated sympathetic response to stress was located in a chromosome 1 QTL for blood pressure (BP) in stroke-prone spontaneously hypertensive rat (SHRSP). In this study, we narrowed down the candidate region to a 1.8-Mbp fragment between D1Rat171 and D1Wox33, and established reciprocal congenic strains for this region. METHODS: Reciprocal congenic strains were established by introgressing the chromosomal segment from SHRSP/Izm into WKY/Izm (Wpch1.21) and vice versa (SPwch1.72). The urinary norepinephrine excretion (u-NE) was quantified with high-performance liquid chromatography in the urine collected under 6 h of cold stress (4 degrees C). ECG was recorded using the telemetry under 3 h of restraint stress, and the relative sympathetic activity was evaluated as the low frequency/high frequency ratio by the power spectral analysis. BP under the stresses was evaluated by the telemetry. RESULTS: The increases in the u-NE during the cold stress and in the low frequency/high frequency ratio under the restraint stress were significantly greater in Wpch1.21 when compared with Wistar-Kyoto (WKY) rat. The increases in BP both under the cold and the restraint stresses were significantly greater in Wpch1.21 than in WKY. In the reciprocal congenic strain, SPwch1.72, the effects of the transferred fragment on the sympathetic stress responses were confirmed as lower u-NE and low frequency/high frequency in this strain than in SHRSP. Further, the BP responses both to the cold and the restraint stresses were significantly greater in SHRSP than in SPwch1.72. CONCLUSION: These results indicated that a small fragment on chromosome 1 harbored a gene (or genes) influencing the sympathetic response to different stresses.

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Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
abnormal response of heart to induced stress  IDA 4889147 RGD 
abnormal response of heart to induced stress susceptibilityIAGPphysical restraint in mechanical restrainer4889147; 4889147compared to SHRSP/Izm in response to restraint stressRGD 
abnormal response of heart to induced stress susceptibilityIAGPphysical restraint in mechanical restrainer4889147; 4889147compared to WKY/Izm in response to restraint stressRGD 
abnormal sensory capabilities/reflexes/nociception  IDA 4889147 RGD 
decreased systemic arterial systolic blood pressure  IAGP 4889147baseline compared to SHRSP/IzmRGD 
decreased systemic arterial systolic blood pressure susceptibilityIAGP 4889147; 4889147baseline compared to SHRSP/IzmRGD 
decreased systemic arterial systolic blood pressure severityIAGPcold ambient air exposure or physical restraint in mechanical restrainer4889147compared to SHRSP/Izm under cold and restraint stressRGD 
decreased urine noradrenaline level  IAGPcold ambient air exposure4889147; 4889147compared to SHRSP/Izm in response to cold stressRGD 
decreased urine noradrenaline level severityIAGPcold ambient air exposure4889147compared to SHRSP/Izm in response to cold stressRGD 
increased circulating noradrenaline level  IDA 4889147 RGD 
increased systemic arterial systolic blood pressure susceptibilityIAGP 4889147; 4889147baseline compared to WKY/IzmRGD 
increased systemic arterial systolic blood pressure susceptibilityIAGPcold ambient air exposure or physical restraint in mechanical restrainer4889147compared to WKY/Izm under cold and restraint stressRGD 
increased urine noradrenaline level  IDA 4889147 RGD 
increased urine noradrenaline level susceptibilityIAGPcold ambient air exposure4889147; 4889147; 4889147compared to WKY/Izm in response to cold stressRGD 
increased urine noradrenaline level susceptibilityIAGPcold ambient air exposure or physical restraint in mechanical restrainer4889147compared to WKY/Izm under cold and restraint stressRGD 


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