RGD Reference Report - Transcriptomic and Innate Immune Responses to Yersinia pestis in the Lymph Node during Bubonic Plague. - Rat Genome Database

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Transcriptomic and Innate Immune Responses to Yersinia pestis in the Lymph Node during Bubonic Plague.

Authors: Comer, JE  Sturdevant, DE  Carmody, AB  Virtaneva, K  Gardner, D  Long, D  Rosenke, R  Porcella, SF  Hinnebusch, BJ 
Citation: Comer JE, etal., Infect Immun. 2010 Dec;78(12):5086-5098. Epub 2010 Sep 27.
RGD ID: 4781452
Pubmed: PMID:20876291   (View Abstract at PubMed)
PMCID: PMC2981309   (View Article at PubMed Central)
DOI: DOI:10.1128/IAI.00256-10   (Journal Full-text)

A delayed inflammatory response is a prominent feature of infection with Yersinia pestis, the agent of bubonic and pneumonic plague. Using a rat model of bubonic plague, we examined lymph node histopathology, transcriptome, and extracellular cytokine levels to broadly characterize the kinetics and extent of the host response to Y. pestis and how it is influenced by the Yersinia virulence plasmid (pYV). Remarkably, dissemination and multiplication of wild-type Y. pestis during the bubonic stage of disease did not induce any detectable gene expression or cytokine response by host lymph node cells in the developing bubo. Only after systemic spread had led to terminal septicemic plague was a transcriptomic response detected, which included upregulation of several cytokine, chemokine, and other immune response genes. Although an initial intracellular phase of Y. pestis infection has been postulated, a Th1-type cytokine response associated with classical activation of macrophages was not observed during the bubonic stage of disease. However, elevated levels of interleukin-17 (IL-17) were present in infected lymph nodes. In the absence of pYV, sustained recruitment to the lymph node of polymorphonuclear leukocytes (PMN, or neutrophils), the major IL-17 effector cells, correlated with clearance of infection. Thus, the ability to counteract a PMN response in the lymph node appears to be a major in vivo function of the Y. pestis virulence plasmid.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL17AHumanplague  ISOIl17a (Rattus norvegicus)mRNA and protein:increased expression:lymph nodeRGD 
Il17aRatplague  IEP mRNA and protein:increased expression:lymph nodeRGD 
Il17aMouseplague  ISOIl17a (Rattus norvegicus)mRNA and protein:increased expression:lymph nodeRGD 

Objects Annotated

Genes (Rattus norvegicus)
Il17a  (interleukin 17A)

Genes (Mus musculus)
Il17a  (interleukin 17A)

Genes (Homo sapiens)
IL17A  (interleukin 17A)


Additional Information