RGD Reference Report - Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc). - Rat Genome Database

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Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc).

Authors: Han, DY  Kang, SR  Park, OS  Cho, JH  Won, CK  Park, HS  Park, KI  Kim, EH  Kim, GS 
Citation: Han DY, etal., Toxicol Ind Health. 2010 Jun;26(5):287-96. Epub 2010 Mar 31.
RGD ID: 4448154
Pubmed: PMID:20356859   (View Abstract at PubMed)
DOI: DOI:10.1177/0748233710364961   (Journal Full-text)

Polychlorinated biphenyls (PCBs) are environmental pollutants that are quite toxic to biological systems. This study examined the inhibitory effect of PCB126 and PCB114 on testicular steroidogenesis in male rats. Male Sprague Dawley rats received weekly intraperitoneal injections of PCB126 (0.2 mg/kg) or PCB114 (20 mg/kg) or vehicle (corn oil). Animals from each group were sacrificed at 2, 5 and 8 weeks after the injections. Blood and testis tissue samples were collected for the hormone assay, Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR). The testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were assayed, and the expression levels of the mRNA and proteins associated with the testosterone biosynthesis pathway were measured to determine the effect of PCB126 and PCB114 on testicular steroidogenesis. The results showed that the testis weight was significantly higher in the PCB126-treated rats given eight shots. Moreover, the serum testosterone levels were significantly lower in the PCB126 and PCB114-treated groups than the control. The transcription and translation levels of P450(17alpha) and P450(scc) were significantly lower in the PCB126-treated groups than the control. These results suggest that PCB126 may affect testicular steroidogenesis by downregulating P450(17alpha), P450(scc) and have inhibitory effect on the testicular functions.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
biphenyl metabolic process  IEP 4448154; 4448154PCB126RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp11a1  (cytochrome P450, family 11, subfamily a, polypeptide 1)
Cyp17a1  (cytochrome P450, family 17, subfamily a, polypeptide 1)


Additional Information