RGD Reference Report - Role of innate immune cells in protection against Toxoplasma gondii at inflamed site. - Rat Genome Database

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Role of innate immune cells in protection against Toxoplasma gondii at inflamed site.

Authors: Nakano, Y  Hisaeda, H  Sakai, T  Zhang, M  Maekawa, Y  Zhang, T  Himeno, K 
Citation: Nakano Y, etal., J Med Invest. 2001 Feb;48(1-2):73-80.
RGD ID: 42724468
Pubmed: (View Article at PubMed) PMID:11286020

The intraperitoneal infection with Toxoplasma gondii (T. gondii) caused accumulation of gamma delta T, NK, NK1.1+T-like (NKT) cells at inflamed sites. To clarify the roles of these cells in protection against T. gondii at the inflamed sites, BALB/c mice were depleted of gamma delta T, NK, NK and NKT cells by treatment with antibody against TCR-gamma delta, asialoGM1 or Interleukin-2 receptor beta-chain (IL-2 R beta), respectively, prior to infection. Mice treated with anti-TCR-gamma delta monoclonal antibody (mAb) became more susceptible to infection, whereas mice treated with anti-IL-2R beta mAb acquired resistance. Treatment with anti-asialoGM1 Ab showed no effect. We previously reported that heat shock protein 65 (HSP65) in macrophages induced by gamma delta T cells plays an essential role in protective immunity against T. gondii infection, by preventing apoptotic death of infected macrophages. In the present study, we showed that treatment with anti-IL-2R beta mAb, but not with anti-asialoGM1 Ab, enhanced the HSP65 induction in macrophages, and inhibited Interleukin-4 (IL-4) expression in nonadherent peritoneal exudate cells. Furthermore, neutralization of endogenous IL-4 by anti-IL-4 mAb enhanced the HSP65 induction in macrophages. These findings suggest that NKT cells, but not NK cells, negatively regulate the protective immunity against T. gondii infection possibly by producing IL-4 and suppressing HSP65 induction.



Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Il2rb  (interleukin 2 receptor subunit beta)

Genes (Mus musculus)
Il2rb  (interleukin 2 receptor, beta chain)

Genes (Homo sapiens)
IL2RB  (interleukin 2 receptor subunit beta)


Additional Information