RGD Reference Report - Expression of proinflammatory genes during estrogen-induced inflammation of the rat prostate. - Rat Genome Database

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Expression of proinflammatory genes during estrogen-induced inflammation of the rat prostate.

Authors: Harris, MT  Feldberg, RS  Lau, KM  Lazarus, NH  Cochrane, DE 
Citation: Harris MT, etal., Prostate. 2000 Jun 15;44(1):19-25.
RGD ID: 4145481
Pubmed: PMID:10861753   (View Abstract at PubMed)

BACKGROUND: Exposure of male Wistar rats to estradiol-17beta (E(2)) in the presence or absence of dihydrotestosterone propionate (DHT) was previously shown to result in prostate inflammation. The present study examines, for the first time, changes in the expression level of several proinflammatory genes during the course of this experimentally induced prostatitis. METHODS: Adult male Wistar rats were given chronic exposure to E(2) + DHT by capsule implantation or were injected with E(2) for short-term exposure. Semiquantitative RT-PCR was employed to measure changes in proinflammatory transcript levels in the separated lobes of the prostate after various times of exposure to estrogen with or without DHT. RESULTS: We observed an upregulation of IL-1beta, IL-6, MIP-2, and inducible nitric oxide synthase (iNOS) after only 4 days treatment with E(2). After 4 weeks of treatment with E(2) + DHT, a significant increase in transcript levels of IL-4, IL-5, IL-6, MIP-2, eotaxin, and iNOS was detected, while IL-1beta and TNF-alpha transcript levels only increased slightly. No increase in transcript levels for cyclooxygenase-2 (cox-2), IFN-gamma, IL-2, or IL-12 was observed. CONCLUSIONS: Upregulation of proinflammatory transcripts occurred shortly after exposure to E(2) and well before any inflammatory cells were observed in the prostate. The pattern of gene expression resembled a T(H)2-type helper-cell response.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
prostatitis  ISOCcl11 (Rattus norvegicus)4145481; 4145481mRNA:increased expression:prostate gland dorsolateral lobeRGD 
prostatitis  IEP 4145481mRNA:increased expression:prostate gland dorsolateral lobeRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
chronic inflammatory response  IEP 4145481 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccl11  (C-C motif chemokine ligand 11)

Genes (Mus musculus)
Ccl11  (C-C motif chemokine ligand 11)

Genes (Homo sapiens)
CCL11  (C-C motif chemokine ligand 11)


Additional Information