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Protective effect of heme oxygenase-1 on lung injury induced by erythrocyte instillation in rats.

Authors: Pang, QF  Zhou, QM  Zeng, S  Dou, LD  Ji, Y  Zeng, YM 
Citation: Pang QF, etal., Chin Med J (Engl). 2008 Sep 5;121(17):1688-92.
Pubmed: (View Article at PubMed) PMID:19024100

BACKGROUND: Intratracheal instillation of blood induces self-repaired acute lung injury. However, the mechanism of repair has been unclear. Heme-oxygenase (HO)-1, which catalyzes heme breakdown, acts as an inducible defense against oxidative stress and plays an important role in inflammation. The objective of this study was to test the role of HO-1 in lung injury caused by intratracheal instillation of red cells. METHODS: Forty healthy, male Sprague-Dawley rats were randomly divided into five groups: normal group, saline group, erythrocyte group, erythrocyte+zinc-protoporphyrin (ZnPP, HO-1 inhibitor) group and saline+ZnPP group. At 2 days after intratracheal instillation of red cells, lung tissues and lavage samples were isolated for biochemical determinations and histological measurements. RESULTS: Histological analysis revealed that administration of ZnPP worsened the acute lung injury induced by instilled erythrocytes. HO-1 was over-expressed in the erythrocyte group and in the erythrocyte + ZnPP group. Compared with the erythrocyte + ZnPP group, the levels of total protein, lactate dehydrogenase and tumor necrosis factor-alpha in the lavage were lower (P < 0.01), while the level of interleukin-10 was higher in the erythrocyte group (P < 0.01). CONCLUSION: HO-1 protects against erythrocyte-induced inflammatory injury in lung.


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RGD Object Information
RGD ID: 4145350
Created: 2010-11-01
Species: All species
Last Modified: 2010-11-01
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.