RGD Reference Report - Elevated expression of surfactant proteins in newborn rats during adaptation to hyperoxia. - Rat Genome Database

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Elevated expression of surfactant proteins in newborn rats during adaptation to hyperoxia.

Authors: White, CW  Greene, KE  Allen, CB  Shannon, JM 
Citation: White CW, etal., Am J Respir Cell Mol Biol. 2001 Jul;25(1):51-9.
RGD ID: 4143429
Pubmed: PMID:11472975   (View Abstract at PubMed)
DOI: DOI:10.1165/ajrcmb.25.1.4296   (Journal Full-text)

The mechanisms whereby lung adaptation to hyperoxia occurs in the newborn period are incompletely understood. Pulmonary surfactant has been implicated in lung protection against hyperoxic injury, and elevated expression of certain surfactant proteins occurs in lungs of adult rats during adaptation to sublethal oxygen (85% O(2)). Here we report that newborn rats, which can adapt to even higher levels of hyperoxia (100% O(2)) than do adult rats, manifest changes in the lung surfactant proteins (SP), especially SP-A and SP-D. In newborn rats exposed to hyperoxia on Days 3 through 10 of life, lung messenger RNAs (mRNAs) for SP-A and SP-B gradually and progressively increased, relative to levels in age-matched, air-exposed newborns, over this 8-d period. By contrast, SP-C and SP-D mRNAs were maximally increased relative to values in simultaneously air-exposed control rats after 4 d of exposure. Lung mRNA for CC-10, a protein specific for Clara cells, was greater in hyperoxia-exposed rats than in air-exposed control rats on Day 4 of exposure, but not on other days. Lung mRNA for thyroid transcription factor (TTF)-1 was marginally increased on Days 1, 2, 4, and 6, and significantly increased on Day 8. Both SP-A and SP-D proteins were increased in lung lavage samples taken from hyperoxia-exposed newborns, relative to those taken from air-exposed controls, with the greatest increases occurring on Days 6 and 8 of exposure. However, the patterns of increase of the proteins were not identical to those of the respective mRNAs. In situ hybridization studies demonstrated increases in SP-D, and to a lesser extent in SP-A, in peripheral lung tissues from oxygen-exposed newborns. Taken together, these data indicate that specific surfactant proteins are upregulated at both the pretranslational and post-translational levels in distal lung epithelium during adaptation to hyperoxia in the newborn rat.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Hyperoxia  ISOSftpa1 (Rattus norvegicus)4143429; 4143429mRNA and protein:increased expression:lungRGD 
Hyperoxia  ISOSftpb (Rattus norvegicus)4143429; 4143429mRNA:increased expression:lungRGD 
Hyperoxia  ISOSftpc (Rattus norvegicus)4143429; 4143429mRNA:increased expression:lungRGD 
Hyperoxia  ISOSftpd (Rattus norvegicus)4143429; 4143429mRNA and protein:increased expression:lungRGD 
Hyperoxia  IEP 4143429; 4143429mRNA and protein:increased expression:lungRGD 
Hyperoxia  IEP 4143429; 4143429mRNA:increased expression:lungRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to hyperoxia  IEP 4143429; 4143429; 4143429; 4143429 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellular space  IDA 4143429; 4143429 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Sftpa1  (surfactant protein A1)
Sftpb  (surfactant protein B)
Sftpc  (surfactant protein C)
Sftpd  (surfactant protein D)

Genes (Mus musculus)
Sftpa1  (surfactant associated protein A1)
Sftpb  (surfactant associated protein B)
Sftpc  (surfactant associated protein C)
Sftpd  (surfactant associated protein D)

Genes (Homo sapiens)
SFTPA1  (surfactant protein A1)
SFTPB  (surfactant protein B)
SFTPC  (surfactant protein C)
SFTPD  (surfactant protein D)


Additional Information