RGD Reference Report - Distinct cytokine production by lung and blood neutrophils from children with cystic fibrosis. - Rat Genome Database

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Distinct cytokine production by lung and blood neutrophils from children with cystic fibrosis.

Authors: Corvol, H  Fitting, C  Chadelat, K  Jacquot, J  Tabary, O  Boule, M  Cavaillon, JM  Clement, A 
Citation: Corvol H, etal., Am J Physiol Lung Cell Mol Physiol. 2003 Jun;284(6):L997-1003. Epub 2003 Jan 24.
RGD ID: 4143175
Pubmed: PMID:12547728   (View Abstract at PubMed)
DOI: DOI:10.1152/ajplung.00156.2002   (Journal Full-text)

Inflammation plays a critical role in lung disease progression in cystic fibrosis (CF). This inflammatory process is dominated by a neutrophil influx in the airways. To determine whether the accumulation of neutrophils in the airways of CF patients is associated with an altered function, we analyzed the capacity of neutrophils isolated from the lung compartment and the blood to release the major neutrophil pro- and anti-inflammatory cytokines IL-8 and IL-1-receptor antagonist (ra) spontaneously and in the presence of LPS. Comparison of cytokine production by blood neutrophils from CF patients and from control subjects showed significantly increased IL-8 and decreased IL-1ra release by CF neutrophils. Comparison of cytokine production by airway and blood neutrophils from CF patients also documented distinct profiles: the spontaneous release of IL-8 and IL-1ra by airway neutrophils was significantly higher than that from blood neutrophils. Culture in the presence of LPS failed to further enhance cytokine production. Analysis of the effect of dexamethasone confirmed the difference in the responsiveness of lung and blood neutrophils in CF. Used at a concentration effective in reducing IL-8 production by blood neutrophils, dexamethasone (10(-6) M) was unable to repress secretion of IL-8 by airway neutrophils. In addition, comparison of cytokine production by airway neutrophils from children with CF and children with dyskinetic cilia syndrome also documented distinct profiles of secretion. These results are consistent with a dysregulated cytokine production by lung and blood neutrophils in CF. They provide support to the hypothesis that not only the CF genotype but also the local environment may modify the functional properties of the neutrophils.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cystic fibrosis  IEP 4143175protein:decreased expression:blood and neutrophilRGD 
cystic fibrosis  ISOIL1RN (Homo sapiens)4143175; 4143175protein:decreased expression:blood and neutrophilRGD 

Objects Annotated

Genes (Rattus norvegicus)
Il1rn  (interleukin 1 receptor antagonist)

Genes (Mus musculus)
Il1rn  (interleukin 1 receptor antagonist)

Genes (Homo sapiens)
IL1RN  (interleukin 1 receptor antagonist)


Additional Information