RGD Reference Report - G protein-coupled receptor kinase 2 and beta-arrestins are recruited to FSH receptor in stimulated rat primary Sertoli cells. - Rat Genome Database

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G protein-coupled receptor kinase 2 and beta-arrestins are recruited to FSH receptor in stimulated rat primary Sertoli cells.

Authors: Marion, S  Kara, E  Crepieux, P  Piketty, V  Martinat, N  Guillou, F  Reiter, E 
Citation: Marion S, etal., J Endocrinol. 2006 Aug;190(2):341-50.
RGD ID: 4140424
Pubmed: PMID:16899567   (View Abstract at PubMed)
DOI: DOI:10.1677/joe.1.06857   (Journal Full-text)

FSH-receptor (FSH-R) signaling is regulated by agonist-induced desensitization and internalization. It has been shown, in a variety of overexpression systems, that G protein-coupled receptor kinases (GRKs) phosphorylate the activated FSH-R, promote beta-arrestin recruitment and ultimately lead to internalization. The accuracy of this mechanism has not yet been demonstrated in cells expressing these different molecules at physiological levels. Using sucrose gradient fractionation, we show that FSH induces the recruitment of the endogenous GRK 2 and beta-arrestin 1/2 from the cytoplasm to the plasma membrane of rat primary Sertoli cells. As assessed by ligand binding, the FSH-R was found expressed in the fractions where GRK 2 and beta-arrestins were recruited upon FSH treatment. In addition, the endogenous beta-arrestin 1 was found dephosphorylated in an agonist-dependent manner. Finally, a significant FSH-binding activity was co-immunoprecipitated with the endogenous beta-arrestins from agonist-stimulated but not from untreated Sertoli cell extracts. This FSH-R interaction with beta-arrestins was sustained for up to 30 min. In conclusion, our data strongly suggest that the GRK/beta-arrestin machinery plays a physiologically relevant role in the regulation of the FSH signaling.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Grk2Ratapical plasma membrane  IDA  RGD 
Arrb1Ratbasolateral plasma membrane  IDA  RGD 
Arrb2Ratbasolateral plasma membrane  IDA  RGD 
Grk2Ratbasolateral plasma membrane  IDA  RGD 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Arrb1  (arrestin, beta 1)
Arrb2  (arrestin, beta 2)
Fshr  (follicle stimulating hormone receptor)
Grk2  (G protein-coupled receptor kinase 2)


Additional Information