RGD Reference Report - Regulation of synaptic structure and function by palmitoylated AMPA receptor binding protein. - Rat Genome Database

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Regulation of synaptic structure and function by palmitoylated AMPA receptor binding protein.

Authors: Misra, C  Restituito, S  Ferreira, J  Rameau, GA  Fu, J  Ziff, EB 
Citation: Misra C, etal., Mol Cell Neurosci. 2010 Apr;43(4):341-52. Epub 2010 Jan 18.
RGD ID: 4107359
Pubmed: PMID:20083202   (View Abstract at PubMed)
PMCID: PMC3061853   (View Article at PubMed Central)
DOI: DOI:10.1016/j.mcn.2010.01.001   (Journal Full-text)

AMPA receptor binding protein (ABP) is a multi-PDZ domain scaffold that binds and stabilizes AMPA receptor (AMPAR) GluR2/3 subunits at synapses. A palmitoylated N-terminal splice variant (pABP-L) concentrates in spine heads, whereas a non-palmitoylated form (ABP-L) is intracellular. We show that postsynaptic Sindbis viral expression of pABP-L increased AMPAR mediated mEPSC amplitude and frequency and elevated surface levels of GluR1 and GluR2, suggesting an increase in AMPA receptors at individual synapses. Spines were enlarged and more numerous and nerve terminals contacting these cells displayed enlarged synaptophysin puncta. A non-palmitoylated pABP-L mutant (C11A) did not change spine density or size. Exogenous pABP-L and endogenous GRIP, a related scaffold, colocalized with NPRAP (delta-catenin), to which ABP and GRIP bind, and with cadherins, which bind NPRAP. Thus postsynaptic pABP-L induces pre and postsynaptic changes that are dependent on palmitoylation and likely achieved through ABP association with a multi-molecular cell surface signaling complex.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Grip2Ratpositive regulation of AMPA glutamate receptor clustering involved_inIMP PMID:20083202UniProt 
Grip2Ratpositive regulation of excitatory postsynaptic potential involved_inIMP PMID:20083202UniProt 
Grip2Ratpositive regulation of neuron maturation involved_inIMP PMID:20083202UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gria1Ratneuron spine  IDA  RGD 
Grip2Ratneuron spine located_inIMP PMID:20083202UniProt 
Grip2Ratneuron spine located_inIDA PMID:20083202UniProt 
SypRatneuron spine located_inIDA PMID:20083202UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Gria1  (glutamate ionotropic receptor AMPA type subunit 1)
Grip2  (glutamate receptor interacting protein 2)
Syp  (synaptophysin)


Additional Information