RGD Reference Report - Effect of Corilagin on the miR-21/smad7/ERK signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse model. - Rat Genome Database

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Effect of Corilagin on the miR-21/smad7/ERK signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse model.

Authors: Yang, Fan  Wang, Yao  Xue, Jun  Ma, Qian  Zhang, Juan  Chen, Yun-Fei  Shang, Zhen-Zhong  Li, Quan-Qiang  Zhang, Shu-Ling  Zhao, Lei 
Citation: Yang F, etal., Parasitol Int. 2016 Aug;65(4):308-15. doi: 10.1016/j.parint.2016.03.001. Epub 2016 Mar 2.
RGD ID: 40925947
Pubmed: PMID:26946098   (View Abstract at PubMed)
DOI: DOI:10.1016/j.parint.2016.03.001   (Journal Full-text)

This study sought to investigate the anti-fibrotic effect of Corilagin via interference with the miR-21/smad7/ERK signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse model. Mice were infected with Schistosoma japonicum cercaria to establish the mouse model of schistosomiasis-induced hepatic fibrosis. At four weeks after infection, the groups were given different medications. The living conditions were observed. Real-time PCR was employed to detect the mRNA levels of miR-21, smad7 and connective tissue growth factor (CTGF), and western blotting was used to examine the protein levels of smad7, CTGF, smad1, p-smad1, smad2, p-smad2, ERK1/2, p-ERK1/2 and TGF-β receptor I. Immunohistochemistry was used to examine the expression of CTGF. Compared with the model group, increasing concentrations of Corilagin improved the quality of life, inhibited the mRNA expression of miR-21, promoted smad7 protein expression, and inhibited CTGF protein expression (p<0.05 or 0.01). Moreover, Corilagin significantly reduced the protein levels of p-smad1, p-smad2, p-ERK1/2, and TGF-β receptor I (p<0.05 or 0.01). CTGF staining in the cytoplasm was markedly decreased by Corilagin (p<0.05 or 0.01). In conclusion, Corilagin inhibited schistosomiasis-induced hepatic fibrosis via the miR21/smad7/ERK pathway in this animal model.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
liver cirrhosis amelioratesISOCcn2 (Mus musculus)40925947; 40925947 RGD 
liver cirrhosis amelioratesIEP 40925947; 40925947 RGD 
liver cirrhosis amelioratesISOMir21a (Mus musculus)40925947; 40925947 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccn2  (cellular communication network factor 2)
Mir21  (microRNA 21)

Genes (Mus musculus)
Ccn2  (cellular communication network factor 2)
Mir21a  (microRNA 21a)

Genes (Homo sapiens)
CCN2  (cellular communication network factor 2)
MIR21  (microRNA 21)


Additional Information