RGD Reference Report - The Gastric Mucosa from Patients Infected with CagA+ or VacA+ Helicobacter pylori Has a Lower Level of Dual Oxidase-2 Expression than Uninfected or Infected with CagA-/VacA- H. pylori. - Rat Genome Database

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The Gastric Mucosa from Patients Infected with CagA+ or VacA+ Helicobacter pylori Has a Lower Level of Dual Oxidase-2 Expression than Uninfected or Infected with CagA-/VacA- H. pylori.

Authors: Li, Hongqian  Zhou, Yunfeng  Zheng, Yufeng  Guo, Hong  Gao, Lei  Chen, Pan  Feng, Dandan  Wu, Lijuan  Yang, Moli  Qi, Yanli  Guo, Hao  Chang, Yongchao  Chu, Fong-Fong  Gao, Qiang 
Citation: Li H, etal., Dig Dis Sci. 2016 Aug;61(8):2328-2337. doi: 10.1007/s10620-016-4144-z. Epub 2016 Apr 5.
RGD ID: 40924640
Pubmed: PMID:27048452   (View Abstract at PubMed)
PMCID: PMC4943970   (View Article at PubMed Central)
DOI: DOI:10.1007/s10620-016-4144-z   (Journal Full-text)


BACKGROUND: Helicobacter pylori (H. pylori) is a well-recognized gastroduodenal pathogen and class I carcinogen. Dual oxidase-2 (DUOX2), a member of NADPH oxidase family, has several critical physiological functions, including thyroid hormone biosynthesis and host mucosal defense.
AIM: To investigate the effect of H. pylori infection on DUOX2 gene expression in human stomach.
MATERIALS AND METHODS: The biopsies were obtained from patients who underwent endoscopic diagnosis. The patient serum was assayed for two virulence factors of H. pylori, CagA IgG and VacA. The inflammation in gastric mucosa was analyzed with histology. Real-time quantitative PCR was used to detect the expression of three members of NADPH oxidase, NOX1, NOX2, and DUOX2, as well as lactoperoxidase (LPO) in the gastric mucosa. NOX2, DUOX2, and myeloperoxidase (MPO) protein levels were quantified by Western blots or immunohistochemistry.
RESULTS: The H. pylori-infected gastric mucosa had more severe inflammation than uninfected samples. However, the expression of DUOX2 mRNA and protein was lower in gastric mucosa of patients with H. pylori infection compared to the uninfected. Among the H. pylori-infected patients, those having CagA IgG or VacA in the serum had lower DUOX2 expression levels than those infected with H. pylori without either virulence factor. The NOX2 and MPO levels were higher in those patients infected with H. pylori irrespective of the virulence factors than those uninfected patients. NOX1 and LPO mRNA were undetectable in the gastric mucosa.
CONCLUSION: CagA+ or VacA+ H. pylori in the stomach of patients may suppress DUOX2 expression to promote its own survival. Increased NOX2 could not eliminate H. pylori infection.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Helicobacter Infections disease_progressionIEP 40924640mRNA and protein:increased expression:mucosa of stomach (human)RGD 
Helicobacter Infections disease_progressionISOCYBB (Homo sapiens)40924640; 40924640mRNA and protein:increased expression:mucosa of stomach (human)RGD 
Helicobacter Infections disease_progressionIEP 40924640mRNA and protein:decreased expression:mucosa of stomach (human)RGD 
Helicobacter Infections disease_progressionISODUOX2 (Homo sapiens)40924640; 40924640mRNA and protein:decreased expression:mucosa of stomach (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cybb  (cytochrome b-245 beta chain)
Duox2  (dual oxidase 2)

Genes (Mus musculus)
Cybb  (cytochrome b-245, beta polypeptide)
Duox2  (dual oxidase 2)

Genes (Homo sapiens)
CYBB  (cytochrome b-245 beta chain)
DUOX2  (dual oxidase 2)


Additional Information