RGD Reference Report - The NF-kappaB p50 subunit is protective during intestinal Entamoeba histolytica infection of 129 and C57BL/6 mice. - Rat Genome Database

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The NF-kappaB p50 subunit is protective during intestinal Entamoeba histolytica infection of 129 and C57BL/6 mice.

Authors: Cho, Kyou-Nam  Becker, Stephen M  Houpt, Eric R 
Citation: Cho KN, etal., Infect Immun. 2010 Apr;78(4):1475-81. doi: 10.1128/IAI.00669-09. Epub 2010 Jan 19.
RGD ID: 40902842
Pubmed: PMID:20086086   (View Abstract at PubMed)
PMCID: PMC2849398   (View Article at PubMed Central)
DOI: DOI:10.1128/IAI.00669-09   (Journal Full-text)

Entamoeba histolytica is the agent of amebic colitis. In this work we examined the intestinal NF-kappaB response to this parasite. Using an enzyme-linked immunosorbent assay (ELISA) and an electrophoretic mobility shift assay, we found that the NF-kappaB subunit p50 predominated in nuclear extracts of whole cecal tissue and of isolated crypts from mice inoculated with E. histolytica. p50 was protective, since C57BL/6 and 129 mice in which there was targeted deletion of this subunit were more susceptible to E. histolytica infection as measured by culture results, cecal parasite ELISA results, and/or histologic scores. The transepithelial electrical resistance of cecal explants from C57BL/6 and 129 p50 knockout mice decreased markedly in response to the parasite compared with the transepithelial electrical resistance of their wild-type counterparts, suggesting that a protective function of p50 was present in the epithelium itself. This work shows that NF-kappaB activity, particularly activity of the p50 subunit, is one factor that contributes to resistance of the gut to E. histolytica infection.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NFKB1HumanEntamoebiasis severityISONfkb1 (Mus musculus) RGD 
Nfkb1RatEntamoebiasis severityISONfkb1 (Mus musculus) RGD 
Nfkb1MouseEntamoebiasis severityIMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nfkb1  (nuclear factor kappa B subunit 1)

Genes (Mus musculus)
Nfkb1  (nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105)

Genes (Homo sapiens)
NFKB1  (nuclear factor kappa B subunit 1)


Additional Information