RGD Reference Report - Proteomic Screen of Brain Glycoproteome Reveals Prion Specific Marker of Pathogenesis. - Rat Genome Database

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Proteomic Screen of Brain Glycoproteome Reveals Prion Specific Marker of Pathogenesis.

Authors: Lamoureux, Lise  Simon, Sharon L R  Waitt, Brooks  Knox, J David 
Citation: Lamoureux L, etal., Proteomics. 2018 Jan;18(1). doi: 10.1002/pmic.201700296.
RGD ID: 40886276
Pubmed: PMID:29087046   (View Abstract at PubMed)
DOI: DOI:10.1002/pmic.201700296   (Journal Full-text)

Transmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders caused by the presence of an infectious prion protein. The primary site of pathology is the brain characterized by neuroinflammation, astrogliosis, prion fibrils, and vacuolation. The events preceding the observed pathology remain in question. We sought to identify biomarkers in the brain of TSE-infected and aged-matched control mice using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). Since the brain proteome is too complex to resolve all proteins using 2D-DIGE, protein samples are initially filtered through either concanavalin A (ConA) or wheat-germ agglutinin (WGA) columns. Four differentially abundant proteins are identified through screening of the two different glycoproteomes: Neuronal growth regulator 1 (NEGR1), calponin-3 (CNN3), peroxiredoxin-6 (Prdx6), and glial fibrillary acidic protein (GFAP). Confirmatory Western blots are performed with samples from TSE-infected and comparative Alzheimer's disease (AD) affected brains and their respective controls from time points throughout the disease courses. The abundance of three of the four proteins increases significantly during later stages of prion disease whereas NEGR1 decreases in abundance. Comparatively, no significant changes are observed in later stages of AD. Our lab is the first to associate the glycosylated NEGR1 protein with prion disease pathology.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NEGR1Humanscrapie  ISONegr1 (Mus musculus) RGD 
Negr1Ratscrapie  ISONegr1 (Mus musculus) RGD 
Negr1Mousescrapie  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Negr1  (neuronal growth regulator 1)

Genes (Mus musculus)
Negr1  (neuronal growth regulator 1)

Genes (Homo sapiens)
NEGR1  (neuronal growth regulator 1)


Additional Information