RGD Reference Report - Evaluation of Placentation and the Role of the Aryl Hydrocarbon Receptor Pathway in a Rat Model of Dioxin Exposure. - Rat Genome Database

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Evaluation of Placentation and the Role of the Aryl Hydrocarbon Receptor Pathway in a Rat Model of Dioxin Exposure.

Authors: Iqbal, Khursheed  Pierce, Stephen H  Kozai, Keisuke  Dhakal, Pramod  Scott, Regan L  Roby, Katherine F  Vyhlidal, Carrie A  Soares, Michael J 
Citation: Iqbal K, etal., Environ Health Perspect. 2021 Nov;129(11):117001. doi: 10.1289/EHP9256. Epub 2021 Nov 8.
RGD ID: 407420268
Pubmed: PMID:34747641   (View Abstract at PubMed)
PMCID: PMC8574979   (View Article at PubMed Central)
DOI: DOI:10.1289/EHP9256   (Journal Full-text)


BACKGROUND: Our environment is replete with chemicals that can affect embryonic and extraembryonic development. Dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), are compounds affecting development through the aryl hydrocarbon receptor (AHR).
OBJECTIVES: The purpose of this investigation was to examine the effects of TCDD exposure on pregnancy and placentation and to evaluate roles for AHR and cytochrome P450 1A1 (CYP1A1) in TCDD action.
METHODS: Actions of TCDD were examined in wild-type and genome-edited rat models. Placenta phenotyping was assessed using morphological, biochemical, and molecular analyses.
RESULTS: TCDD exposures were shown to result in placental adaptations and at higher doses, pregnancy termination. Deep intrauterine endovascular trophoblast cell invasion was a prominent placentation site adaptation to TCDD. TCDD-mediated placental adaptations were dependent upon maternal AHR signaling but not upon placental or fetal AHR signaling nor the presence of a prominent AHR target, CYP1A1. At the placentation site, TCDD activated AHR signaling within endothelial cells but not trophoblast cells. Immune and trophoblast cell behaviors at the uterine-placental interface were guided by the actions of TCDD on endothelial cells.
DISCUSSION: We identified an AHR regulatory pathway in rats activated by dioxin affecting uterine and trophoblast cell dynamics and the formation of the hemochorial placenta. https://doi.org/10.1289/EHP9256.



Objects Annotated

Genes (Rattus norvegicus)
Ahr  (aryl hydrocarbon receptor)
Ahrem1Soar  (aryl hydrocarbon receptor; CRISPR/Cas9 induced mutant1, Soar)

Strains
SD-Ahrem1Soar  (NA)


Additional Information