RGD Reference Report - IL-33 enhances macrophage M2 polarization and protects mice from CVB3-induced viral myocarditis. - Rat Genome Database

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IL-33 enhances macrophage M2 polarization and protects mice from CVB3-induced viral myocarditis.

Authors: Wang, Chao  Dong, Chunsheng  Xiong, Sidong 
Citation: Wang C, etal., J Mol Cell Cardiol. 2017 Feb;103:22-30. doi: 10.1016/j.yjmcc.2016.12.010. Epub 2016 Dec 29.
RGD ID: 40400900
Pubmed: (View Article at PubMed) PMID:28041873
DOI: Full-text: DOI:10.1016/j.yjmcc.2016.12.010

Viral myocarditis is the inflammation caused by myocardial virus infection, and the coxsackievirus group B3 virus (CVB3) is the most common pathogen. An efficient therapeutic agent against viral myocarditis is currently unavailable. IL-33, a new member of the IL-1 cytokine superfamily, exhibits potential immunotherapeutic effect against inflammatory and autoimmune diseases. However, the functional role of IL-33 in viral myocarditis has not been investigated. To examine the therapeutic role of IL-33 in viral myocarditis, an IL-33 overexpression plasmid (pDisplay-IL-33) and IL-33 knockdown plasmid (pLL3.7-IL-33) were packaged with polyethylenimine and delivered intravenously at the orbital area of BALB/c male mice after CVB3 infection. Then, myocarditis severity was assessed 7days after infection. Results showed that IL-33 up-regulation significantly alleviated the severity of viral myocarditis with an increased cardiac contractive function and survival rate. Mechanistic studies demonstrated that IL-33 can stimulate ST2L+F4/80+ macrophages and ST2L+CD4+T cells in cardiac tissue to express IL-4, which is a potent inducer for macrophage M2 polarization. Mice with adoptive transfer of M2 macrophages exhibited less cardiac inflammation and attenuated myocarditis, suggesting the protective role of M2 macrophage in viral myocarditis. Additionally, IL-4 neutralization abolished the IL-33-mediated cardiac functional improvement in myocarditis mice. Collectively, our findings provide a novel therapeutic role for IL-33 in CVB3-induced myocarditis.

Annotation

Disease Annotations    
Viral Myocarditis  (IDA,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Il33  (interleukin 33)

Genes (Mus musculus)
Il33  (interleukin 33)

Genes (Homo sapiens)
IL33  (interleukin 33)


Additional Information