RGD Reference Report - Altered expression of inflammation-related genes in human carotid atherosclerotic plaques. - Rat Genome Database

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Altered expression of inflammation-related genes in human carotid atherosclerotic plaques.

Authors: Di Taranto, Maria Donata  Morgante, Alberto  Bracale, Umberto Marcello  D'Armiento, Francesco Paolo  Porcellini, Massimo  Bracale, Giancarlo  Fortunato, Giuliana  Salvatore, Francesco 
Citation: Di Taranto MD, etal., Atherosclerosis. 2012 Jan;220(1):93-101. doi: 10.1016/j.atherosclerosis.2011.10.022. Epub 2011 Oct 25.
RGD ID: 401959395
Pubmed: PMID:22112851   (View Abstract at PubMed)
DOI: DOI:10.1016/j.atherosclerosis.2011.10.022   (Journal Full-text)


OBJECTIVE: Inflammation is a pivotal process in atherosclerosis development and progression, but the underlying molecular mechanisms remain largely obscure. We have conducted an extensive expression study of atherosclerotic plaques to identify the inflammatory pathways involved in atherosclerosis.
METHODS: We studied 11 human carotid plaques, their respective adjacent regions and 7 control arteries from different subjects. Expression of 92 genes was studied by TaqMan low-density array human inflammation panel. Human aortic endothelial and smooth muscle cells were used for in vitro experiments.
RESULTS: The mRNA levels of 44/92 genes (48%) differed significantly between the tissues examined (13 up-regulated and 31 down-regulated). Dysregulated genes encode molecules belonging to different functional classes although most of them encode enzymes involved in the eicosanoid synthesis pathway. The expression of PTGIS and PTGIR genes was decreased in human aortic endothelial and smooth muscle cells stimulated with oxLDL and TNF-α.
CONCLUSIONS: This study not only reveals several dysregulated genes in human lesions but also focuses the role played by the genes involved in the eicosanoid synthesis pathway during atherosclerotic development. The decrease of PTGIS and PTGIR expression after oxLDL treatment mirrors the decreased mRNA levels in atherosclerotic lesions versus control arteries, which suggests that oxidation is important for PTGIS and PTGIR regulation in human vessel cells during atherosclerosis development.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PTGISHumanatherosclerosis  IEP mRNA:decreased expression:artery (human)RGD 
PtgisRatatherosclerosis  ISOPTGIS (Homo sapiens)mRNA:decreased expression:artery (human)RGD 
PtgisMouseatherosclerosis  ISOPTGIS (Homo sapiens)mRNA:decreased expression:artery (human)RGD 

Gene-Chemical Interaction Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PTGISHumanoxidised LDL decreases expression EXP Oxidized LDL decreases expression of PTGIS mRNA in aortic endothelial cells and aortic smooth muscle cellsRGD 
PtgisRatoxidised LDL decreases expression ISOPTGIS (Homo sapiens)Oxidized LDL decreases expression of PTGIS mRNA in aortic endothelial cells and aortic smooth muscle cellsRGD 
PtgisMouseoxidised LDL decreases expression ISOPTGIS (Homo sapiens)Oxidized LDL decreases expression of PTGIS mRNA in aortic endothelial cells and aortic smooth muscle cellsRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptgis  (prostaglandin I2 synthase)

Genes (Mus musculus)
Ptgis  (prostaglandin I2 (prostacyclin) synthase)

Genes (Homo sapiens)
PTGIS  (prostaglandin I2 synthase)


Additional Information