RGD Reference Report - Cyclooxygenase-1 and bicistronic cyclooxygenase-1/prostacyclin synthase gene transfer protect against ischemic cerebral infarction. - Rat Genome Database

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Cyclooxygenase-1 and bicistronic cyclooxygenase-1/prostacyclin synthase gene transfer protect against ischemic cerebral infarction.

Authors: Lin, Heng  Lin, Teng-Nan  Cheung, Wai-Mui  Nian, Gau-Ming  Tseng, Ping-Hui  Chen, Shu-Fen  Chen, Jean-Ju  Shyue, Song-Kun  Liou, Jun-Yang  Wu, Cheng-Wen  Wu, Kenneth K 
Citation: Lin H, etal., Circulation. 2002 Apr 23;105(16):1962-9. doi: 10.1161/01.cir.0000015365.49180.05.
RGD ID: 401901194
Pubmed: PMID:11997284   (View Abstract at PubMed)
DOI: DOI:10.1161/01.cir.0000015365.49180.05   (Journal Full-text)


BACKGROUND: We tested the hypothesis that bicistronic cyclooxygenase-1 (COX-1)/prostacyclin synthase (PGIS) and COX-1 gene transfer reduce cerebral infarct volume by augmenting synthesis of protective prostaglandins.
METHODS AND RESULTS: We infused into lateral ventricle of a rat stroke model recombinant adenoviruses (rAd) containing COX-1 (Adv-COX-1), COX-1 and PGIS (Adv-COX-1/PGIS), or Adv-PGK control vector, and we determined COX-1 and PGIS protein and eicosanoid levels and infarct volume. COX-1 and PGIS proteins were increased in a time-dependent manner. Adv-COX-1/PGIS infusion selectively augmented prostacyclin levels, with reduction of other eicosanoids in ischemic cortex and a significant reduction of infarct volume, even when the rAd was administered 5 hours after ischemia. Infusion of Adv-COX-1 also increased prostacyclin, suppressed leukotriene levels, and achieved a similar degree of cerebral protection. Its neuroprotection was abrogated by treatment with a selective COX-1 inhibitor.
CONCLUSIONS: COX-1/PGIS and COX-1 gene transfer reduce cerebral infarct volume by augmenting prostacyclin and suppressing leukotriene productions. COX-1-based gene transfer has potential for treating ischemic stroke.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PTGISHumancerebral infarction treatmentISOPtgis (Rattus norvegicus) RGD 
PtgisRatcerebral infarction treatmentIMP  RGD 
PtgisMousecerebral infarction treatmentISOPtgis (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptgis  (prostaglandin I2 synthase)

Genes (Mus musculus)
Ptgis  (prostaglandin I2 (prostacyclin) synthase)

Genes (Homo sapiens)
PTGIS  (prostaglandin I2 synthase)


Additional Information