RGD Reference Report - Expressions of vitamin D metabolic components VDBP, CYP2R1, CYP27B1, CYP24A1, and VDR in placentas from normal and preeclamptic pregnancies. - Rat Genome Database

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Expressions of vitamin D metabolic components VDBP, CYP2R1, CYP27B1, CYP24A1, and VDR in placentas from normal and preeclamptic pregnancies.

Authors: Ma, Rong  Gu, Yang  Zhao, Shuang  Sun, Jingxia  Groome, Lynn J  Wang, Yuping 
Citation: Ma R, etal., Am J Physiol Endocrinol Metab. 2012 Oct 1;303(7):E928-35. doi: 10.1152/ajpendo.00279.2012. Epub 2012 Aug 7.
RGD ID: 401901075
Pubmed: PMID:22871339   (View Abstract at PubMed)
PMCID: PMC3469619   (View Article at PubMed Central)
DOI: DOI:10.1152/ajpendo.00279.2012   (Journal Full-text)

Vitamin D insufficiency/deficiency during pregnancy has been linked to increased risk of preeclampsia. Placenta dysfunction plays an important role in the pathogenesis of this pregnancy disorder. In this study, we tested the hypothesis that disturbed vitamin D metabolism takes place in preeclamptic placentas. Protein expressions of vitamin D binding protein (VDBP), 25-hydroxylase (CYP2R1), 1α-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1), and vitamin D receptor (VDR) were examined in placentas from normotensive and preeclamptic pregnancies. By immunostaining we found that in normal placenta VDBP, CYP24A1, and VDR expressions are localized mainly in trophoblasts, whereas CYP2R1 and CYP27B1 expressions are localized mainly in villous core fetal vessel endothelium. Protein expressions of CYP2R1 and VDR are reduced, but CYP27B1 and CYP24A1 expressions are elevated, in preeclamptic compared with normotensive placentas. Because increased oxidative stress is an underlying pathophysiology in placental trophoblasts in preeclampsia, we further determined whether oxidative stress contributes to altered vitamin D metabolic system in placental trophoblasts. Trophoblasts isolated from normal-term placentas were treated with hypoxic-inducing agent CoCl(2), and protein expressions of VDBP, CYP2R1, CYP27B1, CYP24A1, and VDR were determined. We found that hypoxia-induced downregulation of VDBP, CYP2R1, and VDR and upregulation of CYP27B1 and CYP24A1 expressions were consistent with that seen in preeclamptic placentas. CuZnSOD expression was also downregulated in trophoblasts treated with CoCl(2). These results provide direct evidence of disrupted vitamin D metabolic homeostasis in the preeclamptic placenta and suggest that increased oxidative stress could be a causative factor of altered vitamin D metabolism in preeclamptic placentas.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CYP24A1Humanpre-eclampsia  IEP associated with hypoxia and protein:increased expression:placenta (human)RGD 
CYP27B1Humanpre-eclampsia  IEP associated with hypoxia and protein:increased expression:placenta (human)RGD 
CYP2R1Humanpre-eclampsia  IEP associated with hypoxia and protein:decreased expression:placenta (human)RGD 
Cyp24a1Ratpre-eclampsia  ISOCYP24A1 (Homo sapiens)associated with hypoxia and protein:increased expression:placenta (human)RGD 
Cyp24a1Mousepre-eclampsia  ISOCYP24A1 (Homo sapiens)associated with hypoxia and protein:increased expression:placenta (human)RGD 
Cyp27b1Ratpre-eclampsia  ISOCYP27B1 (Homo sapiens)associated with hypoxia and protein:increased expression:placenta (human)RGD 
Cyp27b1Mousepre-eclampsia  ISOCYP27B1 (Homo sapiens)associated with hypoxia and protein:increased expression:placenta (human)RGD 
Cyp2r1Ratpre-eclampsia  ISOCYP2R1 (Homo sapiens)associated with hypoxia and protein:decreased expression:placenta (human)RGD 
Cyp2r1Mousepre-eclampsia  ISOCYP2R1 (Homo sapiens)associated with hypoxia and protein:decreased expression:placenta (human)RGD 
VDRHumanpre-eclampsia  IEP protein:decreased expression:placenta (human)RGD 
VdrRatpre-eclampsia  ISOVDR (Homo sapiens)protein:decreased expression:placenta (human)RGD 
VdrMousepre-eclampsia  ISOVDR (Homo sapiens)protein:decreased expression:placenta (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp24a1  (cytochrome P450, family 24, subfamily a, polypeptide 1)
Cyp27b1  (cytochrome P450, family 27, subfamily b, polypeptide 1)
Cyp2r1  (cytochrome P450, family 2, subfamily r, polypeptide 1)
Vdr  (vitamin D receptor)

Genes (Mus musculus)
Cyp24a1  (cytochrome P450, family 24, subfamily a, polypeptide 1)
Cyp27b1  (cytochrome P450, family 27, subfamily b, polypeptide 1)
Cyp2r1  (cytochrome P450, family 2, subfamily r, polypeptide 1)
Vdr  (vitamin D (1,25-dihydroxyvitamin D3) receptor)

Genes (Homo sapiens)
CYP24A1  (cytochrome P450 family 24 subfamily A member 1)
CYP27B1  (cytochrome P450 family 27 subfamily B member 1)
CYP2R1  (cytochrome P450 family 2 subfamily R member 1)
VDR  (vitamin D receptor)


Additional Information