RGD Reference Report - Mutant rat phosphatidylinositol/phosphatidylcholine transfer proteins specifically defective in phosphatidylinositol transfer: implications for the regulation of phospholipid transfer activity. - Rat Genome Database

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Mutant rat phosphatidylinositol/phosphatidylcholine transfer proteins specifically defective in phosphatidylinositol transfer: implications for the regulation of phospholipid transfer activity.

Authors: Alb, J G  Gedvilaite, A  Cartee, R T  Skinner, H B  Bankaitis, V A 
Citation: Alb JG, etal., Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8826-30. doi: 10.1073/pnas.92.19.8826.
RGD ID: 39458029
Pubmed: PMID:7568025   (View Abstract at PubMed)
PMCID: PMC41060   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.92.19.8826   (Journal Full-text)

The mammalian phosphatidylinositol/phosphatidylcholine transfer proteins (PI-TPs) catalyze exchange of phosphatidylinositol (PI) or phosphatidylcholine (PC) between membrane bilayers in vitro. We find that Ser-25, Thr-59, Pro-78, and Glu-248 make up a set of rat (r) PI-TP residues, substitution of which effected a dramatic reduction in the relative specific activity for PI transfer activity without significant effect on PC transfer activity. Thr-59 was of particular interest as it is a conserved residue in a highly conserved consensus protein kinase C phosphorylation motif in metazoan PI-TPs. Replacement of Thr-59 with Ser, Gln, Val, Ile, Asn, Asp, or Glu effectively abolished PI transfer capability but was essentially silent with respect to PC transfer activity. These findings identify rPI-TP residues that likely cooperate to form a PI head-group binding/recognition site or that lie adjacent to such a site. Finally, the selective sensitivity of the PI transfer activity of rPI-TP to alteration of Thr-59 suggests a mechanism for in vivo regulation of rPI-TP activity.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
phosphatidylcholine transfer activity enablesIMP 39458029PMID:7568025UniProt 
phosphatidylinositol transfer activity enablesIMP 39458029PMID:7568025UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Pitpna  (phosphatidylinositol transfer protein, alpha)


Additional Information