RGD Reference Report - Synthesis of non-hydroxy-galactosylceramides and galactosyldiglycerides by hydroxy-ceramide galactosyltransferase. - Rat Genome Database

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Synthesis of non-hydroxy-galactosylceramides and galactosyldiglycerides by hydroxy-ceramide galactosyltransferase.

Authors: van der Bijl, P  Strous, G J  Lopes-Cardozo, M  Thomas-Oates, J  van Meer, G 
Citation: van der Bijl P, etal., Biochem J. 1996 Jul 15;317 ( Pt 2):589-97. doi: 10.1042/bj3170589.
RGD ID: 39458006
Pubmed: PMID:8713090   (View Abstract at PubMed)
PMCID: PMC1217527   (View Article at PubMed Central)
DOI: DOI:10.1042/bj3170589   (Journal Full-text)

Galactosylceramide (GalCer) is the major glycolipid in brain. In order to characterize the activity of brain UDPgalactose: ceramide galactosyltransferase (CGalT), it has been stably expressed in CGalT-negative Chinese hamster ovary (CHO) cells. After fractionation of transfected cells, CHO-CGT, on sucrose gradients, the activity resides at the density of endoplasmic reticulum and not of Golgi. A lipid chromatogram from CHO-CGT cells revealed two new iodine-staining spots identified as GalCer, since they comigrate with GalCer standards, can be metabolically labelled with [3H]galactose, are recognized by anti-GalCer antibodies, and are resistant to alkaline hydrolysis. A third [3H]galactose lipid was identified as galactosyldiglyceride. In the homogenate CGalT displays a 25-fold preference for hydroxy fatty acid-containing ceramides. Remarkably, endogenous GalCer of transfected cells contains exclusively non-hydroxy fatty acids: fast atom bombardment and collision-induced dissociation mass spectrometric analysis revealed mainly C16:0 in the lower GalCer band on TLC and mainly C22:0 and C24:0 in the upper band. Our results suggest that CGalT galactosylates both hydroxy- and non-hydroxy fatty acid-containing ceramides and diglycerides, depending on their local availability. Thus, CGalT alone may be responsible for the synthesis of hydroxy- and non-hydroxy-GalCer, and galactosyldiglyceride in myelin.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ugt8Ratgalactosylceramide biosynthetic process involved_inIDA PMID:8713090UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ugt8Ratendoplasmic reticulum located_inIDA PMID:8713090UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ugt8RatN-acylsphingosine galactosyltransferase activity enablesIDA PMID:8713090UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Ugt8  (UDP glycosyltransferase 8)


Additional Information